水溶近紅外熒光染料SF Cy5.5@N3,Sulfo-Cy5.5 N3
Sulfo-Cyanine5.5 is a water-soluble, hydrophilic fluorophore which possesses emission in far red area of the spectrum, an analog of Cy5.5®. As other cyanine dyes, sulfo-Cyanine5.5 has an outstanding molar extinction coefficient which gives rise to its bright fluorescence.
The molecule contains four sulfo groups that provide hydrophilicity and negative charge to the fluorophore - this minimizes non-specific binding.
The azide group of sulfo-Cyanine5.5 azide can be conjugated with terminal alkynes in the presence of copper(I) catalyst, or with cycloalkynes in copper free strain promoted reaction.
General properties：
Appearance: | dark colored solid |
Mass spec M+ increment: | 984.2 |
Molecular weight: | 1099.41 |
Molecular formula: | C43H45K3N6O13S4 |
Solubility: | good in water, DMF, DMSO |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties：
Excitation maximum, nm: | 673 |
ε, L⋅mol−1⋅cm−1: | 195000 |
Emission maximum, nm: | 691 |
CF260: | 0.09 |
CF280: | 0.11 |
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Cy系列的活性基團(tuán)菁染料：
Cyanine3 NHS ester（Cy3@SE）
Cyanine5 NHS ester（Cy5@SE）
Cyanine7 NHS ester（Cy7@SE）
Sulfo Cyanine3 NHS ester（SF Cy3@SE）
Sulfo Cyanine5 NHS ester（SF Cy5@SE）
Sulfo Cyanine7 NHS ester（SF Cy7@SE）
Cy3 Bis NHS ester
Cy5 Bis NHS ester
Cy7 Bis NHS ester
Cyanine3 amine（Cy3@NH2）
Cyanine5 amine（Cy5@NH2）
Cyanine7 amine（Cy7@NH2）
Sulfo Cyanine3 amine（SF Cy3@NH2）
Sulfo Cyanine5 amine（SF Cy5@NH2）
Sulfo Cyanine7 amine（SF Cy7@NH2）
Cyanine3 azide (Cy3@N3)
Cyanine5 azide (Cy5@N3)
Cyanine7 azide (Cy7@N3)
Cyanine3 alkyne (Cy3@alk)
Cyanine5 alkyne (Cy5@Alk)
Cyanine7 alkyne (Cy7@Alk)
Cyanine3 maleimide (Cy3@Mal)
Cyanine5 maleimide (Cy5@Mal)
Cyanine7 maleimide (Cy7@Mal)
Sulfo Cyanine3 azide (SF Cy3@N3)
Sulfo Cyanine5 azide (SF Cy5@N3)
Sulfo Cyanine7 azide (SF Cy7@N3)
Sulfo Cyanine3 alkyne (SF Cy3@Alk)
Sulfo Cyanine5 alkyne (SF Cy5@Alk)
Sulfo Cyanine7 alkyne (SF Cy7@Alk)
Sulfo Cyanine3 maleimide (SF Cy3@MAL)
Sulfo Cyanine5 maleimide (SF Cy5@MAL)
Sulfo Cyanine7 maleimide (SF Cy7@MAL)
