Wnt糖蛋白是存在于多種生物體內的一種細胞外配體，Wnt作為形態發生素通過激發細胞內遠離信號發送區域的濃度依賴反應控制胚胎形態發育。Wnt通路調節動物發育過程中的多個重要環節，例如細胞增殖、細胞遷徙及細胞分化。Wnt蛋白通過自分泌或旁分泌作用與位于細胞膜上的受體相結合，激活細胞內信號通路調節靶基因的表達，對細胞的增殖、分化、遷移、極性化和凋亡起到重要作用。

WNT-5a,human W5A-H-005,5ug 產品簡介
Wnt信號轉導通路因其啟動蛋白為Wnt蛋白而得名，在胚胎發育、細胞增殖及分化等方面發揮重要作用，其異常活化與人類多種腫瘤的發生密切相關。研究顯示，Wnt信號至少存在經典和非經典兩條不同的信號通路，而Wnt-5a屬于非經典Wnt信號通路，既往研究Ror2具有Wnt受體結構域樣結構。有研究認為Wnt-5a經由Ror2介導的信號通路發揮作用。
Human WNT-5a蛋白含有357個氨基酸殘基。

人源WNT-5a 蛋白因子，5ug 產品特性
? 活性在共轉Frizzled- 4 和 LRP- 5基因的HEK293 細胞鑒定，純度高，無動物成分污染
?人源WNT-5a 蛋白因子，5ug 活性在共轉Frizzled- 4 和 LRP- 5基因的HEK293 細胞鑒定
? 人源WNT-5a 蛋白因子，5ug 產品經過SDS-PAGE 和HPLC雙層鑒定純度大于85%

人源WNT-5a 蛋白因子，5ug 保存方法
The lyophilized protein is stable for at least 6 months if stored at -80 degreeC. Reconstituted protein is stable for at least two weeks at 4 degree C, but should be stored in aliquots at -80 degree C for longer term. Avoid repeated freeze and thaw.

產品英文描述
WNT-5a belongs to the class of WNT proteins that activate the “noncanonical” pathway. The predicted size of human WNT-5a is a monomeric protein containing 357 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of ~45 kDa on SDS-PAGE under non-reducing conditions. StemRD’s product WNT-5a,human W5A-H-005,5ug is expressed from a human cell line in animal-free medium, and purified with a proprietary process that is distinct from the published method.
?WNT-5a,human W5A-H-005,5ug Purity: Greater than 85% as determined by SDS-PAGE and HPLC analysis
?WNT-5a,human W5A-H-005,5ug Biological Activity: The activity was determined by using a TCF reporter gene assay in HEK293 cells co-transfected with Frizzled-4 and LRP- 5. WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay (MilkelsAJ, et al., PLoS Biol, 4:e115, 2006). This activation mode is utilized because activation assays are generally more reliable than inhibition assays, as they are less prone to any non-specific inhibitory contamination in the preparat