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當前位置:上海易佰聚經貿有限公司>>以色列Prospec細胞因子>> 重組人骨形態發生蛋白-6(rHuBMP-6)

重組人骨形態發生蛋白-6(rHuBMP-6)

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CYT-441重組人骨形態發生蛋白-6(rHuBMP-6) 2μg/10μg/1mg Prospec

 

 

Background :
 

 
Description :
 Recombinant Human BMP-6 produced in E.Coli is a homodimeric, non-glycosylated, Polypeptide chain containing 142 amino acids and having a molecular mass of 15 kDa. 3 additional amino acids were added from the N-terminal Ala-Pro-Thr to increase bacterial expression.
BMP-6 is purified by proprietary chromatographic techniques.

 
Physical Appearance:
 Sterile Filtered White lyophilized (freeze-dried) powder.

 
Formulation:
 The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.

 
Solubility:
 It is recommended to reconstitute the lyophilized Recombinant BMP-6 in sterile 20mM AcOH (acetic Acid) not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
 Lyophilized Human BMP-6 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution BMP-6 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

 
Purity:
 Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

 
Amino acid sequence:
 The sequence of the first ten N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser-Ala-Ser-Ser-Arg-Arg.

 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of BMP-6.

 
Protein content:
 Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).
2. Analysis by RP-HPLC, using a standard solution of BMP-6 as a Reference Standard.

 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


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