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當前位置：上海易佰聚經貿有限公司>>以色列Prospec細胞因子>> 重組人骨形態發生蛋白-6（rHuBMP-6）
重組人血小板生成素TPO/Thrombopoietin CHO
| CYT-441 | 重組人骨形態發生蛋白-6（rHuBMP-6） | 2μg/10μg/1mg | Prospec |
| Background : | |
 | Description : |
| Recombinant Human BMP-6 produced in E.Coli is a homodimeric, non-glycosylated, Polypeptide chain containing 142 amino acids and having a molecular mass of 15 kDa. 3 additional amino acids were added from the N-terminal Ala-Pro-Thr to increase bacterial expression. BMP-6 is purified by proprietary chromatographic techniques. | |
| Physical Appearance: |
| Sterile Filtered White lyophilized （freeze-dried） powder. | |
| Formulation: |
| The protein was lyophilized from a concentrated （1mg/ml） sterile solution containing no additives. | |
| Solubility: |
| It is recommended to reconstitute the lyophilized Recombinant BMP-6 in sterile 20mM AcOH （acetic Acid） not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
| Stability: |
| Lyophilized Human BMP-6 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution BMP-6 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles. | |
| Purity: |
| Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. | |
| Amino acid sequence: |
| The sequence of the first ten N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser-Ala-Ser-Ser-Arg-Arg. | |
| Endotoxin: |
| Less than 0.1 ng/µg （IEU/µg） of BMP-6. | |
| Protein content: |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of BMP-6 as a Reference Standard. | |
| Usage: |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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