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當前位置:上海易佰聚經貿有限公司>>以色列Prospec細胞因子>> 重組人骨形態發生蛋白2(rHuBMP-2)

重組人骨形態發生蛋白2(rHuBMP-2)

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CYT-261重組人骨形態發生蛋白2(rHuBMP-2) 2μg/10μg/1mg Prospec

 

 

Background :
 Belongs to the Bone-growth regulatory factors that are members of the transforming growth factor-beta (TGF-beta) superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.
 
Description :
 Recombinant Human Bone Morphogenetic protein-2 produced in E.Coli is a homodimeric, non-glycosylated, Polypeptide chain containing 115 amino acids and having a molecular mass of 26018 Dalton.
BMP-2 is purified by proprietary chromatographic techniques.
 
Physical Appearance:
 Sterile Filtered White lyophilized (freeze-dried) powder.
 
Formulation:
 BMP-2 was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM sodium citrate pH=3.5.
 
Solubility:
 It is recommended to reconstitute the lyophilized rhBMP-2 in sterile 20mM AcOH (acetic Acid) not less than 100µg/ml, which can then be further diluted to other aqueous solutions
 
Stability:
 Lyophilized BMP-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution BMP-2 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
 
Purity:
 Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
 
Amino acid sequence:
 The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Ala-Lys-His.
 
Dimers and aggregates:
 Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
 
Biological Activity:
 ProSpec's BMP-2 is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of MC3T3-E1 cells is less then 50 ng/ml.
 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of Bone Morphogenetic protein-2 .
 
Protein content:
 Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).
2. Analysis by RP-HPLC, using a standard solution of BMP-2 as a Reference Standard.
 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


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