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| CYT-284 | 重組大鼠血管內皮生長因子相關蛋白（rrVEGF-C152） | 2μg/10μg/1mg | | Prospec |
| Background: | | | VEGF-C152S is a point mutant generated by the replacement of the second conserved Cys residue of the recombinant processed VEGF-C by a Ser residue. VEGF-C 152S is analog to the human VEGF-C 156S mutant and only active toward VEGFR-3/FLT-4 but, unlike wild type VEGF-C, is unable to bind to and to activate signalling through VEGFR-2/KDR. VEGF-C152S was inactive in the vascular permeability assay and did not increase migration of the capillary endothelial cells, indicating that these VEGF-like effects of VEGF-C require VEGFR-2 binding. VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein （VRP）, is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The rat VEGF-C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant rat VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT -4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant rat VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A.
|  | Description : | | |
recombinant rat VEGF-C 152 contains 152 amino acids residues and was fused to a His-tag （6x His） at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions.
| | Physical Appearance: | | | Sterile Filtered White lyophilized （freeze-dried） powder.
| | Formulation: | | | The protein was lyophilized from a concentrated （1mg/ml） solution with BSA.
| | Solubility: | | | It is recommended to reconstitute the lyophilized Recombinant Rat VEGF-C 152 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
| | Stability:
| | | Lyophilized Rat VEGF-C152 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution VEGF-C 152 should be stored at 40C between 2-7 days and for future use below -180C.
Please prevent freeze-thaw cycles.
| | Purity: | | | Greater than 90.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Anion-exchange FPLC.
（c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
| | Dimers and aggregates: | | | Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
| | Biological Activity: | | | Measured by its ability to stimulate phosphorylation of the VEGFR-3/FLT-4 receptor in porcine aortic endothelial cells （PAE/FLT -4 cells）. The ED50 for this effect is typically 150-300 ng/ml, corresponding to a specific activity of 5 x 103 Units/mg.
| | Endotoxin: | | | Less than 0.1 ng/µg （IEU/µg） of Rat VEGF-C152.
| | Protein content: | | | Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of VEGF-C152 as a Reference Standard.
| | Usage: | | | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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佰易聚生物商城提供美國光譜醫學透析袋，wako生化試劑，amresco生化試劑，sigma生化試劑，優質的分子生物學試劑和試劑盒、培養基本和培養耗材、細胞因子和細胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現貨火爆*,歡迎登錄 www.ebioeasy.com或者,，洽談選購！
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