[ INTENDED USE ]
The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TGFb1 in human serum,
plasma, tissue homogenates, cell culture supernates and other biological fluids.
[ REAGENTS AND MATERIALS PROVIDED ]
Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard （lyophilized） 2 Standard Diluent 1×20mL
Detection Reagent A （green） 1×120μL Assay Diluent A （2 × concentrate） 1×6mL
Detection Reagent B （red） 1×120μL Assay Diluent B （2 × concentrate） 1×6mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer （30 × concentrate） 1×20mL Instruction manual 1
[ MATERIALS REQUIRED BUT NOT SUPPLIED ]
1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution

[ STORAGE OF THE KITS ]
1. For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection
Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20oC upon receipt while the
others should be at 4 oC.
2. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above
storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and
reseal along entire edge of zip-seal.