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產品英文名稱 | Mouse BMP-4 Protein, His tag |
產品中文名稱 | 重組小鼠骨形態發生蛋白4,His-標簽(BMP-4) |
別名 | bone morphogenetic protein 4, BMP-2B, DVR4, BMP4 |
表達宿主 | 大腸桿菌 |
種屬 | 小鼠 |
序列 | 氨基酸序列來源于:小鼠BMP-4 (P21275) (Lys303-Arg408)表達的蛋白片段。 |
活性 | Testing in progress. |
蛋白長度 | 重組小鼠BMP-4由106個氨基酸組成,預測分子量為12.1KD。 |
純度 | > 98% ,使用SDS-PAGE檢測 |
特點&優勢 | 通過LAL法測定,每微克蛋白里內毒素含量<0.1>0.1> |
產品形式 | 凍干粉,凍干緩沖液為無菌的PBS,pH 4.0。 |
基因ID | 12159 |
蛋白序列鏈接 | /uniprotkb/P21275/entry#sequences |
使用中注意事項 | 開蓋使用前,請先離心。本產品為凍干粉,推薦用該產品配套的Reconstitution Buffer進行復溶,且濃度不低于100 µg/mL。客戶可根據后續的實驗需求,進行進一步的稀釋。 |
保存建議 | 本產品為凍干粉形式,建議:將凍干粉產品保存于-20℃以下的干燥環境中。本產品溶解之后,可在4℃保存2-7天,長期使用請置于-20℃以下。為長期保存,推薦添加載體蛋白(0.1% HSA或者BSA),并酌情分裝保存,避免反復凍融。 |
運輸條件 | 冰袋運輸(藍冰) |
警告 | 本文列出的產品僅供研究使用,不適用于人類或臨床診斷。我們產品所推薦應用,不是建議使用我們的產品去違反任何或許可證。對于使用本產品可能發生的侵權或其他違規行為,我們不承擔任何責任。 |
背景 | Bone morphogenetic protein 4 (BMP4) is a multifunctional ligand protein belonging to TGF β Family. BMP4 is expressed by endothelial cells (EC) in response to hypoxia and promotes proliferation of vascular smooth muscle cells (SMC). For example, it inhibits the proliferation of SMCs isolated from the proximal pulmonary artery while inducing the proliferation of SMCs isolated from the distal pulmonary artery. BMP4 seems to be a marker and driver of vascular calcification, especially in atherosclerosis. BMP4 induces angiogenesis, endothelial cell (EC) proliferation, and migration. BMP4 is differentially expressed in calcified atherosclerotic plaque, which is the junction between atherosclerotic vascular calcification and normal bone formation mechanism. BMP4 increases plaque formation, promotes oxidative stress, endothelial dysfunction and osteogenic differentiation through its proinflammatory and atherogenic effects. |
Fig.Mouse BMP-4 Protein, His tag