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產品英文名稱 | Anti-His Tag Rabbit Polyclonal Antibody |
產品中文名稱 | His標簽兔多克隆抗體 |
別名 | His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag |
克隆類型 | 多克隆 |
檢測類型 | WB |
宿主 | 兔 |
免疫原 | 合成多肽 |
產品形式 | 液體形式 |
存儲緩沖液 | PBS緩沖液(pH 7.4),含有0.5%BSA(穩定劑),0.02%(防腐劑)和50%甘油。 |
保存建議 | 自發貨之日起,-20°C可穩定保存1年。為限度的避免損失,請在打開管蓋之前融化抗體并離心。我們建議使用前分裝以避免反復凍融。 |
運輸條件 | 冰袋運輸(藍冰) |
警告 | 本文列出的產品僅供研究使用,不適用于人類或臨床診斷。我們產品所推薦應用,不是建議使用我們的產品去違反任何或許可證。對于使用本產品可能發生的侵權或其他違規行為,我們不承擔任何責任。 |
背景 | A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems. |
Fig. Western blot analysis of His fusion protein in cell lysate from transfected 293T cells (lane A) and baculovirus expression vector system (lane B) at 1:2000 dilutions.