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商品信息

產品英文名稱	Mouse G-CSF protein
產品中文名稱	重組小鼠粒細胞集落刺激因子（G-CSF)
別名	G-CSF; Colony Stimulating Factor 3; Granulocyte; GCSF

商品屬性

標簽	無標簽
表達宿主	大腸桿菌
種屬	小鼠
序列	氨基酸序列來源于：重組小鼠粒細胞集落刺激因子（G-CSF) (P09920) (V31-A208) 表達的蛋白片段。
活性	通過其誘導M-NFS-60細胞增殖的能力來測定。ED50為2.038 pg/mL。
蛋白長度	重組小鼠粒細胞集落刺激因子（G-CSF) 由178個氨基酸組成，預測分子量為18.95 KD。
純度	> 99% ，使用SDS-PAGE檢測
特點&優勢	通過LAL法測定，每微克蛋白里內毒素含量<1.0>
產品形式	凍干粉，凍干緩沖液為無菌的20 mM Tris，150 mM NaCl，pH 7.4。
基因ID	12985
蛋白序列鏈接	/uniprotkb/P09920/entry
使用中注意事項	開蓋使用前，請先離心。本產品為凍干粉，推薦用該產品配套的Reconstitution Buffer進行復溶，且濃度不低于100 µg/mL。客戶可根據后續的實驗需求，進行進一步的稀釋。
保存建議	本產品為凍干粉形式，建議：將凍干粉產品保存于-20℃以下的干燥環境中。本產品溶解之后，可在4℃保存2-7天，長期使用請置于-20℃以下。為長期保存，推薦添加載體蛋白（0.1% HAS/BSA或12%FBS），并酌情分裝保存，避免反復凍融。
運輸條件	冰袋運輸（藍冰）
警告	本文列出的產品僅供研究使用，不適用于人類或臨床診斷。我們產品所推薦應用，不是建議使用我們的產品去違反任何或許可證。對于使用本產品可能發生的侵權或其他違規行為，我們不承擔任何責任。

附加信息

背景

Granulocyte-colony stimulating factor (G-CSF) is a growth factor and an essential cytokine belonging to the CSF family of hormone-like glycoproteins. It is produced by numerous cell types including immune and endothelial cells. G-CSF binding to its receptor G-CSF-R which belongs to the cytokine receptor type I family depends on the interaction of alpha-helical motifs of the former and two fibronectin type III as well as an immunoglobulin-like domain of the latter. Recent animal studies have also revealed that G-CSF activates multiple signaling pathways, such as Akt and also the Janus family kinase-2 and signal transducer and activation of transcription-3 (Jak2-STAT3) pathway, thereby promoting survival, proliferation, differentiation and mobilisation of haematopoietic stem and progenitor cells. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem cell mobilizer and erythropoietin as a cytoprotective agent. G-CSF prevents left ventricular remodeling after myocardial infarction by decreasing cardiomyocyte death and by increasing the number of blood vessels, suggesting the importance of direct actions of G-CSF on the myocardium rather than through mobilization and differentiation of stem cells. Accordingly, recombinant human (rh)G-CSF has been extensively used in clinical haematology and oncology to enable bone marrow transplantation or to treat chemotherapy-associated neutropenia. In preclinical study, G-CSF improved cardiac function and perfusion by angiomyogenesis and protection of cardiomyocytes in myocardial infarction.

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