別稱

SNU387; NCI-SNU-387

種屬

人類

年齡（性別）

女性，41歲

組織來源

肝癌

生長特性

貼壁細胞

細胞形態

上皮細胞樣

背景描述

SNU-387 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus a combination of doxorubicin and mitomycin-C. Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat-inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat inactivated fetal bovine serum.

生物安全等級

2

生長培養基

RPMI-1640＋10% FBS＋1% P/S

推薦傳代比例

1:3-1:6

推薦換液頻率

2~3次/周

倍增時間

~61小時 (PubMed=7543080)

凍存條件

凍存液：55% 基礎培養基+40%FBS+5%DMSO溫度：液氮

培養條件

氣相：空氣，95%；CO2，5%溫度：37℃

保藏機構

ATCC; CRL-2237