成人做爰免费视频免费看_成人a级高清视频在线观看,成人a大片在线观看,成人a大片高清在线观看,成人av在线播放,一a一级片,一级黄 中国色 片,一级黄 色蝶 片,一级黄色 片生活片

武漢原生原代生物醫藥科技有限公司

PriCells: Primary brain cell isolation and culture.

時間:2022-1-18 閱讀:258
分享:

PriCells: Primary brain cell isolation and culture.                                                      


1. Cerebella were removed from 7-day-old mice and passed through Nitex nylon netting (80 μm pore size) into primary cell system containing 20% (v/v) fetal calf serum (FCS).

2. The cell suspension was plated at a density of four cerebella per 12-well culture plate.

3. Primary cell culture system was changed two days after plating and twice a week thereafter, gradually decreasing the FCS concentration to 10%.

4. At 14 days in culture, dibutyryl-cAMP was added to the medium for 1 week to promote the morphological differentiation of astrocytes.

5. Experiments were performed on 3-week-old cultures.

6. Cortical astrocytes were prepared from 1-day-old mice following the same procedure used for cerebellar astrocytes plated at a density of two cortices per 12-well culture plate.

7. Cerebellar neurons were isolated and cultured from the cerebellum of 7-day-old mice, after mild trypsinization of the tissue followed by trituration in a DNase solution containing a trypsin inhibitor derived from soybeans.

8. Cells were suspended in a slightly modified primary cell culture containing 50 μM kainic acid and 10% (v/v) FCS and plated at density 2 × 106 cells per well in a 12-well culture plate.

9. The wells had been coated with poly-L-lysine.

10. Cytosine arabinoside (20 μM) was added after 48 h to prevent astrocyte proliferation.

11. Cells were used for experiments after 1 week in culture.

12. For the co-cultures, the neurons and astrocytes were prepared separately and seeded as described earlier.

13. The astrocytes were cultured on inserts for 21 days, and the neurons were cultured in culture plates for 7 days prior to incubation in co-culture.

14. The astrocytes were grown on permeable membranes in tissue culture plate inserts, and the neurons were cultured in standard tissue culture plates.

15. The inserts were transferred to the neuronal plates 24 h prior to incubation.

16. At time of insert transfer, half of the neuronal medium was extracted and replaced with astrocyte cell culture system.

會員登錄

×

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

X
該信息已收藏!
標簽:
保存成功

(空格分隔,最多3個,單個標簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復您~
撥打電話 產品分類
在線留言
主站蜘蛛池模板: 国产欧美一区二区精品忘忧草| 久久久久精品噜噜久久久| 亚洲人成人网毛片在线播放| 一边做一边说国语对白| 亚洲男人午夜天堂| 亚洲欧美日韩国产精品| 国产免费A片好硬好爽好深小说| 亚洲伊人色综合网色欲WWW| 亚洲中文欧美日韩| 国产精品久久久久久久福利竹| 又大又黄又爽免费看A片| 人妻洗澡被强公日日澡电影| 性一交一乱一伦一A片| 精品久久久久久久久久久好| 久久精品国产亚洲aaaa| 免费观看高清在线视频 国产| 久久精品无码专区免费青青| 欧美区亚洲区国产区| 欧美一区二区三区精品久久| 91forin吃瓜网今日吃瓜 热门大瓜| 亚洲欧美日韩久久久| 国产精品久久久久无码AV1| 免费sm性奴虐视频网站| 日产日韩亚洲欧美综合搜索| 欧美人和另类×xz0z0| 亚洲中文字幕网址在线| 人妻中文字幕乱人伦在线| 亚洲AV鲁丝一区二区三区| 久久精品国产只有精品96| bl肉文推荐失禁| 色欲AV久久一区二区三区久| 久久久久久久成人精品| 久久精品欧美日韩精品不卡| 久久人妻精品国产| 九九热这里只精品| 蜜臀AV性色A片在线观看| 欧美日韩国产精品| 久久夜色撩人精品国产av| 国产永久一区二区三区| 高h 纯肉文bg| FREECHINESE内射少妇|