成人做爰免费视频免费看_成人a级高清视频在线观看,成人a大片在线观看,成人a大片高清在线观看,成人av在线播放,一a一级片,一级黄 中国色 片,一级黄 色蝶 片,一级黄色 片生活片

武漢原生原代生物醫(yī)藥科技有限公司

PriCells: Primary Culture of Pulmonary Arterial Smooth Muscle Cells

時(shí)間:2021-11-11 閱讀:240
分享:

PriCells: Primary Culture of Pulmonary Arterial Smooth Muscle Cells                            

1.Primary cultures of Pulmonary Arterial Smooth Muscle Cells (PASMCs) were isolated from human main pulmonary artery.
2.A segment of the main pulmonary artery was excised and placed in a sterile 10-cm dish containing primary cell culture media.
3.The segment was stripped of adventitia with a sterile forceps.
4.The main pulmonary artery segment was then cut longitudinally to open the vessel, and the endothelial layer was removed by gentle rubbing with a cell scraper.
5.The vessel was then cut into 2-mm segments, inverted, and placed on a collagen-coated 35-mm tissue culture dish.
6.A drop of primary cell culture media containing 10% fetal bovine serum, primary cell culture supplements, antibiotics, and antimycotics was then added, and the cells were grown overnight at 37°C in a humidified atmosphere with 5% CO2-95% air.
7.The next day, an additional 2 ml of complete medium were added.
8.The growth medium was subsequently changed every 2 days.
9.When SMC islands could be observed under the microscope, the tissue segment was removed and the individual cell islands were subcloned.
10.Identity was confirmed as PASMCs by immunostaining (>99% positive) with SMC actin. This was taken as evidence that cultures were not contaminated with fibroblasts or endothelial cells.
11.All cultures for subsequent experiments were maintained in primary cell culture media containing 10% fetal bovine serum, primary cell culture supplements, antibiotics, and antimycotics at 37°C in a humidified atmosphere with 5% CO2-95% air.

References
1.Chamley-Campbell, J., Campbell, G., and Ross, R.: The Smooth Muscle in Cell Culture, Physiol. Res. 59, 1, 1979.
2.Richardson, M., Taylor, D., Casey, M., MacDonald, P., and Stull, J.: Biochemical Markers of Contraction in Human Myometrial Smooth Muscle Cells in Culture, In Vitro Cell. Dev. Biol. 23, 21, 1987.
3.Eskin, S., Sybers, H., Lester, J., Navarro, L., Gotto, A., and DeBakey, M.: Human Smooth Muscle Cells Cultured From Atherosclerotic Plaques and Uninvolved Vessel Wall, In Vitro 17 (8), 713, 1981

會(huì)員登錄

×

請(qǐng)輸入賬號(hào)

請(qǐng)輸入密碼

=

請(qǐng)輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~
撥打電話 產(chǎn)品分類
在線留言
主站蜘蛛池模板: 美女裸露100%奶头视频| 午夜亚洲国产理论片4080| 日韩内射美女人妻一区二区三区| 国产精品扒开腿做爽爽爽视频| 美女翘臀强进入系列在线观看| 欧美 日韩 亚洲一区| 两个吃奶一个添下面视频| 欧美又黑又大AAA毛片| 日韩av综合色区人妻| 欧美日韩理论片在线观看| 国产亚洲精品精华液| 国产精品天干天干在线| 九哥草逼网| 亚洲欧美日韩在线二区| 影音先锋 av天堂| 国 产 自偷自拍网站| 免费观看在线视频国产| 在线视频一区二区三区在线播放| 韩国r级限制电影2018推荐| 色悠久久久久久久综合网| 亚洲色婷婷久久精品AV蜜桃久久| 日韩欧美激情四射| 在线观看视频国产一区| 国产又色又爽又黄的视频免费观看| 爆乳女神玩具酱护士| 日韩欧美在线激情| 久久久久久久久在热| 色噜噜狠狠色综无码久久合欧美| 久久热这里只有精品6| 日本成本人精品无码国产| 国产视频精品在线偷拍| 欧美激情亚洲一区二区三区| 久久精品亚洲国产AV涩情| 漂亮人妻bd中字| 国产日韩欧美一区在线| 亚洲免费av一区二区三区| 亚洲精品v天堂中文字幕| 污全彩肉肉无遮挡彩色| 国产精品野外AV久久久| 国产精品天干天干在线观看| 久久久久久久网|