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Biomatrica公司DNAgard® Blood Tubes
閱讀：2447 發(fā)布時間：2018-3-27Biomatrica公司DNAgard® Blood Tubes
Biomatrica授權(quán)蘇州螞蟻淘生物科技有限公司代理銷售旗下產(chǎn)品，
Biomatrica開發(fā)出一種新型的核心技術(shù)，旨在防止復(fù)雜的樣品和試劑在運輸、存儲以及處理過程中的降解，從而使科學(xué)家們能獲得更多重復(fù)性和可靠的實驗結(jié)果，提率，節(jié)省時間。Biomatrica目前的產(chǎn)品穩(wěn)定核酸并防止降解，而無需冷藏
More cost effective than EDTA
- Optimized for DNA methylation, PCR and NGS assays
- Preserve and Maintain Sample Integrity for 3 months
- Compatible with multiple extraction/purification techniques
- Compatible with all downstream applications
Overview
DNAgard® Blood Tube is designed for the collection, preservation and shipping of whole blood samples for genomic DNA isolation. The preservative reagent rapidly permeates cellular structures and membranes (cell lysis) to stabilize and protect genomic DNA in whole blood samples. The use of DNAgard Blood Tube provides a streamlined workflow from blood collection in the clinic or field to sample processing in the laboratory.
Collect, ship and preserve DNA in blood under optimal conditions
Recover high quality DNA (high MW fragments)
Recover quantitative DNA (yield)
Product Data
Protect DNA at ambient temperatures
Figure-1: DNAgard Blood Tube formulation preserves DNA in whole blood for 3 months. Gel images of isolated genomic DNA recovered from blood samples preserved for 14 months in DNAgard Blood (DGB) at room temperature, in a competitor preservative (COM) at room temperature, unprotected (NP) at room temperature, versus control (-20°C).
Broad range of applications
DNA protected using the DNAgard Blood Tube formulation is suitable for use in many downstream applications, e.g. long-range PCR, real time PCR, etc.
Figure 2A . Successful Long-range PCR after 2 months storage. Genomic DNA isolated from human whole blood stored for 60 days in the DNAgard Blood Tube formulation, non-protected (NP) or frozen at -20°C. Long-range PCR amplification of a 22 Kbp amplicon was performed on the DNA (M = 1 kb ladder). Figure 2B. Real-time PCR after 1 month storage. Genomic DNA isolated from human whole blood stored for 30 days in the DNAgard Blood Tube formulation or frozen at -20°C was quantified using real-time PCR amplification of the b-actin gene.
Representative DNA yield
DNA protected using the DNAgard Blood Tube formation is suitable for use in many downstream applications, e.g. long-range PCR, real time PCR, etc.
Figure 3. qPCR quantification after 1 month storage of blood, from 3 different donors, in DNAgard Blood Tube formulation (RT), Competitor (RT) or frozen (-20°C).
Specifications
Evacuated, sterile, blood collection tubes (10ml)
Pre-fill volume (preservative) = 1.0ml
Blood draw volume = 6.5ml
Storage temperature = -20°C to 45°C
Blood DNA preservation = 3 months at room temperature
For Research Use Only. Not for use in diagnostic procedures.