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沈振國：NMT證實豌豆L3變種分生/伸長/成熟區Cd吸收弱于ZM變種
NMT作為生命科學底層核心技術，是建立活體創新科研平臺的*技術。2005年~2020年，NMT已扎根中國15年。2020年，中國NMT銷往瑞士蘇黎世大學，正式打開歐洲市場。
研究使用平臺：NMT重金屬創新科研平臺
期刊：Journal of Hazardous Materials
主題：NMT證實豌豆L3變種分生/伸長/成熟區Cd吸收弱于ZM變種
標題：The root iron transporter 1 governs cadmium uptake in Vicia sativa roots
影響因子：7.65
檢測指標：Cd2+流速
檢測樣品：豌豆
Cd2+流實驗處理方法：
1）10μmolL-1 Cd預處理10分鐘后，檢測距離根尖0、0.1、0.2、2、5、10、20mm的根上的點
2）+Cd、+Cd+Fe環境下，檢測Cd2+流
Cd2+流實驗測試液成份：
0.1 mmol L−1 KCl, 0.2 mmol L−1 Na2SO4, and 0.3 mmol L−1 MES, at pH 5.8
作者：南京農業大學沈振國、夏妍、張興興
中文摘要（谷歌機翻）
鎘是植物的非必需元素，會抑制植物的生長和發育。豌豆的ZM變種比L3變種對Cd毒性更敏感，但其潛在機理尚不*清楚。
在這里，我們證明根系原生質體中根部Cd含量和Cd熒光強度顯示，其Cd積累量高于L3。VsRIT1是ZIP（ZRT / IRT樣蛋白）家族的成員，在暴露于Cd的情況下，其ZM根的表達水平比L3根高8倍。VsRIT1表達增加了擬南芥和酵母中Cd的運輸和積累。這表明VsRIT1參與了苜蓿根系對鎘的吸收。
此外，由于ZM中較高的RIT1表達，當單獨暴露于Cd或同時暴露于Cd和鐵（Fe）時，ZM根尖具有比L3根更高的瞬時Cd流入能力。我們的發現還表示，鎘可能會與鐵或鋅競爭通過VsRIT1吸收到豌豆或酵母中。
Fig. 6 Fe reduced Cd influx in root tips in ZM and L3. （A)）The transient Cd fluxes of ZM and L3 roots were determined over 10 min after 10 μmol L−1 Cd pre-treatment for 10 min at different distances from the root apex. Negative values indicate Cd influx. (B and C) Fe supply prevents transient Cd influx in both L3 and ZM root tips, columns labelled with distinct lowercase letters indicate statistically significant differences among treatments.
英文摘要
Cadmium is a non-essential element for plants and that inhibits plant growth and development. The ZM variety of Vicia sativa is more sensitive to Cd toxicity than that L3 variety, but the underlying mechanism is not fully understood.
Here, we demonstrated that showed higher Cd accumulation than L3 based on root Cd content and Cd fluorescence intensity in root protoplasts. VsRIT1, a member of ZIP the (ZRT/IRT-like protein) family, showed expression levels in ZM roots 8-fold higher than those in L3 roots under Cd exposure. VsRIT1 expression increased Cd transport and accumulation in Arabidopsis and yeasts. This suggests that VsRIT1 participates in Cd uptake by V. sativa roots.
Furthermore, ZM root tips have a higher capacity for transient Cd influx than L3 roots when exposed to Cd alone or Cd and iron (Fe) together, owing to the higher RIT1 expression in ZM. Our findings also imply that Cd may compete with Fe or/and zinc (Zn) for uptake via VsRIT1 in V. sativa or yeasts.