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Plant Cell 南農(nóng)：硫化氫參與調(diào)節(jié)ABA誘導(dǎo)氣孔關(guān)閉
NMT是基因功能的活體檢測(cè)技術(shù)，已被103位諾貝爾獎(jiǎng)得主所在單位，及北大、清華、中科院使用。
Plant Cell | 南京農(nóng)業(yè)大學(xué)謝彥杰研究組揭示硫化氫參與調(diào)節(jié)ABA誘導(dǎo)氣孔關(guān)閉的分子機(jī)制
期刊：Plant Cell
主題：硫化氫參與調(diào)節(jié)ABA誘導(dǎo)氣孔關(guān)閉的分子機(jī)制
標(biāo)題：Persulfidation-based Modification of Cysteine Desulfhydrase and the NADPH Oxidase RBOHD Controls Guard Cell Abscisic Acid Signaling
影響因子：8.631
檢測(cè)指標(biāo)：H2O2流速
檢測(cè)樣品：擬南芥保衛(wèi)細(xì)胞
H2O2流實(shí)驗(yàn)處理方法：
4周齡的擬南芥幼苗，10μM ABA / 100μM NaHS瞬時(shí)處理
H2O2流實(shí)驗(yàn)測(cè)試液成份：10 mM MES，10 mM KCl，pH 6.15
作者：南京農(nóng)業(yè)大學(xué)謝彥杰、沈杰、張晶、周明健、周恒
中文摘要（谷歌機(jī)翻）
越來(lái)越多的證據(jù)表明，硫化氫（H2S）是一種氣體信號(hào)分子，通過(guò)過(guò)硫化作用來(lái)調(diào)節(jié)多種細(xì)胞信號(hào)傳導(dǎo)途徑，這涉及特定半胱氨酸殘基的翻譯后修飾（PTM）以形成過(guò)硫化物。但是，這種基于氧化還原的重要PTM的機(jī)理在高等植物中仍然知之甚少。
因此，我們已經(jīng)分析了蛋白質(zhì)過(guò)硫化如何在植物脫落酸（ABA）反應(yīng)過(guò)程中作為一種特定且可逆的信號(hào)轉(zhuǎn)導(dǎo)機(jī)制起作用。在這里，我們顯示ABA以氧化還原依賴(lài)性方式刺激Cys44和Cys205的L-半胱氨酸脫硫酶1（DES1）（一種重要的內(nèi)源H2S酶）的過(guò)硫化作用。此外，可持續(xù)的H2S積累會(huì)驅(qū)動(dòng)Cys825和Cys890上NADPH氧化酶呼吸爆發(fā)氧化酶同源蛋白D（RBOHD）的過(guò)硫化，從而增強(qiáng)其產(chǎn)生活性氧的能力。在生理上，S-過(guò)硫化誘導(dǎo)的RBOHD活性與ABA誘導(dǎo)的氣孔關(guān)閉有關(guān)。
這些過(guò)程共同形成一個(gè)負(fù)反饋回路，可微調(diào)保衛(wèi)細(xì)胞氧化還原穩(wěn)態(tài)和ABA信號(hào)傳導(dǎo)。這些發(fā)現(xiàn)不僅擴(kuò)展了我們目前在保衛(wèi)細(xì)胞ABA信號(hào)傳導(dǎo)方面對(duì)H2S功能的認(rèn)識(shí)，而且還證明了涉及特定的和可逆的基于氧化還原的PTM的快速信號(hào)整合機(jī)制的存在，該機(jī)制是響應(yīng)不斷變化的環(huán)境條件而發(fā)生的。
(B) ABA- and NaHS-induced net H2O2 influxes in guard cells of rbohD and pCAB3:RBOHD rbohD plants. Leaves from 4-week-old plant were preincubated for 3 hr in opening buffer (10 mM MES, pH 6.15, and 10 mM KCl) under light (120 μE m−2s−1) and washed in MES buffer three times for 15 min each. The net H2O2 influxes were observed after ABA (10 μM) or NaHS (100 μM) treatment during the indicated times (n = 6)
英文摘要
Accumulating evidence suggests that hydrogen sulfide (H2S) is a gaseous signaling molecule that regulates diverse cellular signaling pathways through persulfidation, which involves the post translational modification (PTM) of specific cysteine residues to form persulfides. However, the mechanisms that underlie this important redox-based PTM remain poorly understood in higher plants.
We have, therefore, analyzed how protein persulfidation acts as a specific and reversible signaling mechanism during the plant abscisic acid (ABA) response. Here we show that ABA stimulates the persulfidation of L-cysteine desulfhydrase 1 (DES1), an important endogenous H2S enzyme, at Cys44 and Cys205 in a redox-dependent manner. Moreover, sustainable H2S accumulation drives persulfidation of the NADPH oxidase respiratory burst oxidase homolog protein D (RBOHD) at Cys825 and Cys890, which enhance its ability to produce reactive oxygen species. Physiologically, S-persulfidation-induced RBOHD activity is relevant to ABA-induced stomatal closure.
Together, these processes form a negative feedback loop that fine-tunes guard cell redox homeostasis and ABA signaling. These findings not only expand our current knowledge of H2S function in the context of guard cell ABA signaling, but also demonstrate the presence of a rapid signal integration mechanism involving specific and reversible redox-based PTMs that occur in response to changing environmental conditions.