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Column : C18 100mm*4.6mm, 3µm
Flow Rate: 1.5ml/min
Wave Length : 280nm
Temperature: 25°C
Runtime : 20 minutes
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當(dāng)前位置：北京豫維科技有限公司>>技術(shù)文章>>多潘立酮與氟哌利多的分離-Zodiac C18 色譜柱
多潘立酮與氟哌利多的分離-Zodiac C18 色譜柱
Column : C18 100mm*4.6mm, 3µm
Flow Rate: 1.5ml/min
Wave Length : 280nm
Temperature: 25°C
Runtime : 20 minutes
1.) : 流動(dòng)相A
Dissolve 5.0g of Ammonium Acetate in 1000ml of HPLC Grade Water
2.) 流動(dòng)相 : B
Methanol HPLC Grade
3.) Gradient Program :
| Time (min) | 流動(dòng)相-A(%V/V) | 流動(dòng)相- B(%V/V) |
| 0-10 | 70 | 30 |
| 10-15 | 0 | 100 |
| 15-15.01 | 70 | 30 |
| 15.01-20 | 70 | 30 |
4.) Diluent : N,N Dimethylformamide
Domperidone Reference Solution (a) : (0.10 and 0.15 mg/ml) Weight accurately about 10mg of Domperidone CRS and 15mg of Droperidol CRS into a 100ml Volumetric flask dissolve and dilute to volume with Diluent and mix Inject 10µl of Domperidone Reference Solution (a) in duplicate and calculate Resolution between Domperidone and Droperidol.
Acceptance Criteria:
Resolution between Domperidone and Dropridol should not be less than 2.0
Column Name : Zodiac C18 Column
Compound Name : Droperidol, Domperidone
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