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iRT – indexed Retention Time – is a dimensionless value that defines chromatographic retention of a peptide for a defined resin type (e.g. C18) relative to the iRT Standard. In contrast to empirical retention time, the iRT value of a peptide is stable and enables accurate prediction of peptide retention for any chromatographic setup.
The iRT Standard contains eleven non-naturally occurring synthetic peptides in a pooled mix. Peptides have been carefully optimized for stability, sensitivity and even retention time spacing over the gradient. The iRT Kit allows straightforward determination of peptide iRT values and calibration of chromatographic systems.
By calibrating your LC system with the iRT Kit you will dramatically increase the throughput of your targeted proteomics (MRM and PRM) experiments – even up to 20 times – by bundling more transitions into one run. The benefits of iRT standards are also important in the next-generation proteomic workflows such as DIA or SWATH. iRT peptides allow high-quality spectral library generation and precise normalization of your DIA or SWATH runs.
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