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ScienCell ECM 1001內皮細胞培養基說明書
閱讀：17647發布時間：2017-12-5
產品描述
ScienCell 1001 ECM內皮細胞培養基是經滅菌的液體培養基，包含必需和非必需氨基酸、維生素、有機和無機化合物、激素、生長因子（ECGS\ECGF）、微量礦物質和低濃度胎牛血清(5%)。內皮細胞培養基（ECM）緩沖體系為重碳酸鹽，在含5%CO2的細胞培養箱中平衡后pH值為7.4。內皮細胞培養基（ECM）的配方能夠選擇性的促進正常人類微血管內皮細胞體外培養中的增殖和生長，并為其達到營養平衡狀態提供數量上和質量上的保證。
產品組份
500mL基礎培養基，25mL FBS，5mL內皮細胞生長因子（ECGS），5mL penicillin/streptomycin solution。
注意：FBS, ECGS 和P/S沒有預混好。要配制完整的ECM培養基需要分別添加。
使用
Sciencell 1001 內皮細胞培養基僅供科研使用。
儲存
基礎培養基：4℃。ECGS, FBS and P/S 溶液：-20℃。避光。
運輸
基礎培養基：室溫。添加劑：干冰。
使用說明：
ECGS, FBS and P/S 溶液37℃溶解。加入培養基前試管彈撥幾次使內容物充分溶解。培養基瓶子和試管用70%酒精消毒。無菌環境中，開蓋時注意不要讓手指碰到內壁。添加ECGS, FBS and P/S 到培養基并且充分混勻。由于多種成分是光不穩定的，培養基要避光使用。使用前不推薦用37℃水浴加熱培養基。避光4℃保存時，重新配制的培養基能穩定放置1個月。
注意：如果沒有正確使用，培養基的某些成分會影響健康。處理時做好防護措施，比如穿防護衣和防護鏡。
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英文原版
Product Description
Endothelial Cell Medium (ECM), when used with Endothelial Cell Growth Supplement (ECGS,
Cat #1052) and 25 ml of fetal bovine serum (FBS) is a complete medium designed for optimal
growth of normal endothelial cells in vitro. It is a sterile, liquid medium which contains essential
and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth
factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is
HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an
atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to
provide an optimally balanced nutritional environment that selectively supports the growth of
normal endothelial cells in vitro.
Components
ECM consists of 500 ml of basal medium, 25 ml of FBS (Cat. #0025), 5 ml of Endothelial Cell
Growth Supplement (ECGS, Cat. #1052) and 5 ml of penicillin/streptomycin solution (P/S, Cat.
#0503). Note: FBS, ECGS and P/S are not pre-mixed in ECM; they must be added separay to
make the complete ECM.
Product Use
ECM is for research use only. It is not approved for human or animal use, or for application in in
vitro diagnostic procedures.
Storage
Store the basal medium at 4oC and the ECGS, FBS and P/S solution at -20oC. Protect from light.
Shipping
Basal medium: room temperature. Supplements: dry ice.
Instructions for Use
Thaw ECGS, FBS and P/S solution at 37oC. Gently tilt the tubes several times to ensure the contents are compley mixed before adding to the medium. Spray the medium bottle and tubes with 70% ethanol, and wipe to remove excess liquid. In a sterile field, remove the caps without touching the interior threads with fingers. Add ECGS, FBS and P/S solution to the medium and mix well. Since several components are light-labile, the medium should not be exposed to light for extended periods. We do not recommend warming medium in a 37oC water bath prior to use.When stored in the dark at 4oC, the reconstituted medium is stable for one month.
Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.
ScienCell ECM 1001內皮細胞培養基說明書中文版由紅榮微再編輯翻譯。
ScienCell ECM 1001內皮細胞培養基訂購信息
品牌 | 貨號 | 產品描述 | 包裝 |
ScienCell | 1001 | 內皮細胞培養基包含500ml基礎培養基，25ml胎牛血清，5ml內皮細胞生長因子和5 ml青霉素/鏈霉素溶液 | Kit |
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