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POPN-048-磷酸酶測(cè)試盒 pNPP Phosphatase Assay Kit
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- 2025年5月12日
- 2025年11月12日
- 北京北京市
- 美國(guó)
- 252次
【產(chǎn)品簡(jiǎn)介】
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磷酸酶測(cè)試盒
pNPP Phosphatase Assay Kit
應(yīng)用分類 酶活性測(cè)定
測(cè)定方法 比色法（405nm）定量測(cè)定磷酸酶活性。
Description Para-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at 405 nm on a spectrophotometer.This homogeneous "mix-and-measure" assay involves simply adding a single reagent to the phosphatase and measuring the product formation on an absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37°C.
Key features High sensitivity and wide linear range. The detection limit is generally 3 ng phosphatase or below.Homogeneous and simple procedure. No wash or reagent transfer steps are involved. The assay can be completed within 30 minutes.Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.
Applications Enzyme Activity Assay and Quality Control for phosphatase production. Characterization of Kinetics of phosphatase reaction. Drug Discovery: high-throughput screen for phosphatase inhibitors.
Storage conditions. The kit is shipped at ambient temperature. Store Reagent at -20°C and other components at 4°C. Shelf life: 12 months.
產(chǎn)品信息
貨號(hào) | 產(chǎn)品名稱 | 規(guī)格 | 單價(jià)（元） |
POPN-048 | 磷酸酶測(cè)試盒 | 48T | 329.65 |
POPN-500 | 磷酸酶測(cè)試盒 | 500T | 1179.9 |