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英文名稱 anti-FLAG Tag Rabbit Polyclonal antibody
應用 WB 1:2500 IF 1:100 IP 1:100 Optimal dilutions should be determined by the end user.
規 格 100ul
濃 度 1mg/ml
保 存 Store at -20°C. Do not aliquot the antibody.
儲存液 PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol
純化方法 affinity purified by Protein A
亞 型 IgG
性 狀 Liquid
克隆類型 polyclonal
宿 主 Rabbit
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
說明書
Anti- Flag Tag Rabbit Polyclonal Antibody
Catalog No. RG001060
Size 100μl
Storage: Store at -20°C. Do not aliquot the antibody.
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to
interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively
as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to
separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can
also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many
different assays that require recognition by an antibody. If there is no antibody against the studied protein,
adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag
sequence.
Molecular Weight: N/A
Application: WB 1:2500 IF 1:100 IP 1:100 Optimal dilutions should be determined by the end user.
Specificity: The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag
fusion proteins.
Form: Liquid , 1mg/ml
Storage buffer: PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol
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RG001060 Anti- Flag Tag Rabbit Polyclonal Antibody
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