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Kanamycin Sulfate、Kanamycin A、硫酸卡那霉素
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產(chǎn)品介紹
KANAMYCIN MONOSULFATE 硫酸卡那霉素
Product Description（產(chǎn)品描述） Kanamycin sulfate is an antimicrobial agent effective against Gram-negative and Gram-positive bacteria and mycoplasma. It binds to the 70S ribosomal subunit, inhibits translocation, and elicits miscoding. Cross-resistance occurs between kanamycin and neomycin, framycetin, and paromomycin, and partial cross-resistance has been reported between kanamycin and streptomycin. Aminoglycosidemodifying enzymes （acetyltransferase, phosphotransferase, nucleotidyltransferase） alter the antibiotic, preventing its interaction with ribosomes. |
Applications（應(yīng)用） Kanamycin Sulfate used in biotechnology applications to inhibit protein synthesis. Active against many strains of Gram-negative bacteria and Gram positive Staphylococcus aureus and S. epidermis. Some strains of Mycobacterium are sensitive. Most active in alkaline solution. |
Analysis NoteOnce added to the agar plate and the plates are not sealed from moisture kanamycin sulfate is prone to degradation. |
Biochem/physiol Actions（生化/生理作用） Kanamycin is an aminoglycoside antibiotic. Aminoglycosides work by binding to the bacterial 30S ribosomal subunit, causing misreading of t-RNA, leaving the bacterium unable to synthesize proteins vital to its growth. Specifically Kanamycin binds to four nucleotides of 16S rRNA and a single amino acid of protein S12. This interferes with decoding site in the vicinity of nucleotide 1400 in 16S rRNA of 30S subunit. This region interacts with the wobble base in the anticodon of tRNA. This leads to interference with the initiation complex, misreading of mRNA so incorrect amino acids are inserted into the polypeptide leading to nonfunctional or toxic peptides and the breakup of polysomes into nonfunctional monosomes. |
Recommended Use（推薦使用） Add the appropriate amount of powder to water while mixing （not the reverse）, as adding water to the powders may result in insoluble mixtures that cannot be filtered without losing potency. If desired, the pH may be adjusted, but a basal media with greate |
Suitability（適應(yīng)性） Suitable for cell culture |
Synonyms（別名） Kanamycin A; Kanamycin sulfate salt
CAS Number 25389-94-0
Grade Cell Culture Reagent
Molecular Formula（分子式） C18H36N4O11 . H2SO4
Molecular Weight（分子量） 582.6
Physical Appearance（外觀） White Powder
Storage & Handling（儲存及處理）Store at Room Temperature （15-30 °C）.
Typical Working Concentration 100 mg/L
Ordering information（訂購信息）
貨號 | 名稱 | 產(chǎn)地 | 規(guī)格 | 報價/元 | */元 |
KANAMYCIN MONOSULFATE 硫酸卡那霉素 | MPBIO | 5g | 272 | 212 | |
60206ES10 | Kanamycin Sulfate 硫酸卡那霉素 | Yeasen | 10G | 169 | 169 |
