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Jem Biotech KTS-01說明書
KlenTaq-S酶——適PAP聚合酶
PAP(焦磷酸化激活的聚合反應法)是一種*的DNA擴增技術（Liu and Sommer: BioTechniques, 29 [2000], 1072-1080; Nucleic Acids Res, 30 [2002], 598-604),該技術利用DNA聚合酶在3'封閉的引物進行連續的焦磷酸化和聚合反應。焦磷酸化是DNA聚合的逆反應，其引物模板雙鏈的3'末端封閉核苷酸（通常是雙脫氧）在無機焦磷酸鹽存在下被酶解清除，在焦磷酸化清除封閉的核苷酸后，激活引物進行后續的DNA聚合。由于PAP的高特異性（1 / 3*1011），該技術正成為檢測存在大量野生型等位基因的情況下檢測稀有突變，大雜合缺失和重復基因的有力工具（Liu et al：Biotechniques， 40 [2006]，661-668）。 理論上，PAP可以檢測3*1011個拷貝的野生型等位基因中的單堿基突變，此外，PAP還可用作替代或證實real-time PCR反應 (Boon et al : Prenat Diagn 27[2007], 932-937)。
KlenTaq-S酶：
KlenTaq-S已被多個研究團隊證實是PAP反應適的熱穩定Taq DNA聚合酶，該酶由5'缺失和F667Y（Tabor和Richardson）突變的基因表達，編碼的Thermus aquaticus DNA聚合酶。 該酶反復暴露于98度的PR1反應緩沖液中沒有表現出任何明顯的活性損失，具有*的熱穩定性。
KlenTaq-S酶的濃度 : 10 U/µl
建議PAP反應條件 (25 µl反應體系) :
50 mM Tris-HCl, 7.8,
16 mM 硫酸銨,
3.5 mM 氯化鎂,
25 µM each four dNTPs,
0.1 µM 引物,
5-200 ng模板,
90 µM 無機焦磷酸鹽 (Na4PPi),
2% DMSO,
循環條件 :
95度1 min (變性),
94度 15 sec
60度 30 sec
64度 30 sec
68度 1 min
72度 1-2 min
循環數25-45 。
緩沖體系 (10X PR1):
500 mM Tris-HCl, pH 7.8,
160 mM (NH4)2SO4,
35 mM MgCl2, 1.5 mg/ml BSA
訂貨信息 :
貨號 : KTS-01
含量: 100 µl or 1000 U/vial
價格 : $200/vial (1000 U)
jembio主要產品：
Item Code | Products | Host | Amount | Price ($) |
LA001 | Acid Phosphatase, Polyclonal anti-Human Affinity Purified | Host Chicken | 5 mg | 1300 |
LA002 | Albumin, Polyclonal anti-Human Affinity Purified (ALB) | Host Goat | 10 mg | 800 |
LA003 | Albumin, Polyclonal anti-Human Affinity Purified (ALB) | Host Chicken | 10 mg | 600 |
LA004 | Alpha Amylase, Polyclonal anti-Human (whole antiserum) | Host Rabbit | 50 ml | 900 |
LA005 | Alpha Fetoprotein, Polyclonal anti-Human (AFP) Whole Antiserum | Host Goat | 50 ml | 900 |
LA006 | Alpha-1 Antitrypsin, Polyclonal anti-Human Affinity Purified (A1AT) | Host Goat | 10 mg | 700 |
LA007 | Alpha-1 Antitrypsin, Polyclonal anti-Human Affinity Purified (A1AT) | Host Chicken | 10 mg | 700 |
LA008 | Alpha-1-Acid Glycoprotein, anti-Human Affinity Purified (A1AG) | Host Goat | 10 mg | 750 |
LA009 | alpha-2-Macroglobulin, Polyclonal anti-Human affinity Purified (A2M) | Host Chicken | 10 mg | 700 |
LA010 | anti-Feline Leukemia Virus p27, Monoclonal | Tissue Culture | 5 mg | 3000 |
LA011 | anti-Feline Leukemia Virus p27, Monoclonal-CMI | Tissue Culture | 5mg | 3000 |
LA012 | beta 2-Microglobulin ,anti-Human HRP Conjugated | Host Chicken | 10 mg | 1400 |
LA013 | beta 2-Microglobulin, Polyclonal anti-Human Affinity Purified (B2M) | Host Chicken | 10 mg | 650 |
LA014 | BNP Polyclonal anti-human affinity purified | Host Rabbit | 5 mg | 3000 |
LA015 | C-Reactive Protein (CRP), Polyclonal anti-Rat affinity purified | Host Rabbit | 1 mg | 1500 |
LA016 | C-Reactive Protein, Polyclonal anti-Human affinity purified (CRP) | Host Goat | 10 mg | 750 |
LA017 | C3, Polyclonal anti-Human Affinity Purified | Host Chicken | 10 mg | 750 |
LA018 | C4 , Polyclonal anti-Human Affinity Purified | Host Goat | 10 mg | 750 |
LA019 | Cathepsin, Polyclonal anti-Human Affinity Purified | Host Chicken | 5 mg | 1450 |
LA020 | Ceruloplasmin, Polyclonal anti-Human Affinity Purified | Host Chicken | 10 mg | 750 |
LA021 | Elastase, anti-Human IgG Fraction | Host Sheep | 100 mg | 500 |
LA021 | Elastase, anti-Human IgG Fraction | Host Sheep | 100 mg | 500 |
LA022 | Elastase, Polyclonal anti-human (whole anti-serum) | Host Sheep | 30 ml | 400 |
LA023 | Ferritin, Polyclonal anti-Human Affinity Purified | Host Rabbit | 10mg | 1750 |
LA024 | Goat anti-Human Whole Serum | Host Goat | 100 ml | 1750 |
LA025 | Haptoglobin Polyclonal anti-human (whole antiserum) | Host Goat | 10 ml | 500 |
LA026 | Haptoglobin, Polyclonal anti-Human Affinity Purified (Hp) | Host Goat | 10 mg | 1200 |
LA027 | Haptoglobin, Polyclonal anti-Human Affinity Purified (Hp) | Host Rabbit | 10 mg | 1200 |
LA028 | Heartworm,.Polyclonal anti-Canine Affinity Purified | Host Chicken | 10 mg | 3000 |
LA029 | Heartworm,Polyclonal anti-Canine,HRP Conjugated | Host Chicken | 10 mg | 3500 |
LA030 | Hemoglobin, Polyclonal anti-Human Affinity Purified | Host Goat | 30 ml | 750 |
LA031 | Hemoglobin, Polyclonal anti-Human Affinity Purified | Host Sheep | 10 mg | 750 |
LA032 | IgA, Polyclonal anti-Human Affinity Purified | Host Chicken | 10 mg | 750 |
LA033 | Kappa, Polyclonal anti-Human Affinity Purified | Host Chicken | 10 mg | 1000 |
LA034 | Kappa, Polyclonal anti-Human Affinity Purified | Host Goat | 10 mg | 750 |
LA035 | Lactate Dehydrogenase , Polyclonal anti-Human Affinity Purified (LDH) | Host Chicken | 10 mg | 3500 |
LA036 | Lactoferrin Polyclonal anti-Human Affinity Purified | Host Goat | 10 mg | 1300 |
LA037 | Lactoferrin, Polyclonal Whole anti-serum | Host Rabbit | 50 ml | 1000 |
LA038 | Lambda, Polyclonal anti-Human Affinity Purified | Host Chicken | 10 mg | 1000 |
LA039 | Lambda, Polyclonal anti-Human Affinity Purified | Host Goat | 10 mg | 750 |
LA040 | Myeloperoxidase, Polyclonal anti-human affinity purified | Host Chicken | 10 mg | 3500 |
LA041 | Myeloperoxidase, Polyclonal anti-Human affinity purified (MPO) | Host Rabbit | 10 mg | 3500 |
LA042 | Myoglobin Polyclonal anti-human affinity purified | Host Chicken | 10 mg | 2500 |
LA043 | Myoglobin, Polyclonal anti-Human Affinity Purified | Sheep | 10 mg | 5000 |
LA044 | Prostate Specific Antigen,(PSA) Polyclonal anti-Human Affinity Purified | Host Chicken | 5 mg | 2300 |
LA045 | Rabbit anti-Human Whole Serum | Host Rabbit | 50 ml | 2000 |
LA046 | Retinol Binding Protein ,Polyclonal anti-Human Affinity Purified (RBP) | Host Chicken | 10 mg | 3200 |
LA047 | Transferrin, Polyclonal anti-Human Affinity Purified | Host Chicken | 10 mg | 750 |
LA048 | Troponin I, anti-human whole antiserum | Host Rabbit | 500 ml | 15000 |
MMT001 | α His tag, monoclonal |
| Inquire | Inquire |
MMT002 | α Taq Pol, monoclonal |
| Inquire | Inquire |
MMT003 | α KlenTaq Pol, monoclonal |
| Inquire | Inquire |
MMT004 | α HIV P24, monoclonal |
| Inquire | Inquire |
MMT005 | α CD3, monoclonal |
| Inquire | Inquire |
MMT006 | α CD4, monoclonal |
| Inquire | Inquire |
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