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簡述

血管搏動力學刺激培養單艙生物反應器，流體剪應力、流體壓力模擬血流流動血管剪切應力培養,機械應力體外三維培養,應力環境下構建腸管尿管管道細胞組織工程。

lumegen生物反應器的培養艙產生搏動和剪切應力刺激到一個單一的血管構建。

標準構建的尺寸范圍從2-6毫米內直徑和從10到60毫米長。

模塊化設計可以方便的安裝支架和細胞種植獨立的模擬脈動刺激系統

多孔的開口，方便導管探頭插入，樣品的采集，或其他各種傳感器的插入。

這個可以高壓滅菌的培養艙的容積只有60毫升,節省了大量的營養液與昂貴的生長因子等.

LumeGen培養艙使用不銹鋼接接頭固定血管或心血管支架和其他需要模擬力學刺激的管狀組織。
標準接口尺寸包括2，3，4，5和6mm外徑,可以選擇更大管徑的接頭.

LumeGen生物反應器系統有收縮舒張壓模擬搏動培養。

這個模擬脈動培養功能是用20或40牛頓直線電機，重量輕，結構緊湊，耐腐蝕，可以長時間工作在培養箱的環境下.
 

詳解：

LumeGen Vascular Bioreactor , TGT's DynaGen Series

 

Watch a video of the LumeGen bioreactor.

The DynaGen® Series bioreactors provide mechanical stimulation to three dimensional tissue engineered constructs to create physiological conditions in-vitro. The LumeGen system imparts pulsatile flow and pressure to a vascular conduit. Applications include the investigation of cell function and differentiation, pharmaceutical benchtop testing, and the seeding and growth of engineered tissues and medical devices.

Chambers
The LumeGen bioreactor chamber creates pulsatile and shear stress stimulation for a single vascular construct. Standard construct sizes may range from 2 to 6mm inner diameter and from 10 to 60mm in length. The modular design facilitates scaffold installation and cell seeding separate from the stimulation system. Multiple ports are available for media access, catheter probe insertion, sample collection, or further instrumentation. The autoclavable chamber has a volume of 60mL. Additional chamber features include:

Grip Options
The LumeGen chambers use barbed stainless steel fittings with an elastic clamp to imobilize the construct. Standard barb sizes accommodate 2, 3, 4, 5 and 6mm lumen internal diameters and are interchangeable using a universal connector system.

DynaGen® Stimulator:
The LumeGen bioreactor system includes the 6Vr pulsatile pressure mechanical stimulator. The 6Vr stimulator features a 40N linear motor that is compact, corrosion resistant and incubator compatible. The stimulator employs pressure control to deliver user defined sinusoidal or physiological pressure waveforms to a maximum pressure of 200 mmHg. The 6Vr is integrated with a computer controlled mean flow pump and is capable of low or high-flow configurations. The flow rate of the mean flow pump is automatically altered to maintain the desired mean pressure within the heart valve. The stimulator dimensions are as follows:

GrowthWorks® Software & Control:
A fifth generation design, the GrowthWorks Software and Control platform includes advanced capabilities, such as multiple waveform control, data acquisition and multi-motor operation. The intuitive software runs on a Windows® laptop computer and features a simple user interface. User defined stimulation profiles are controlled by the software and are readily monitored using graphical displays. Integrated data acquisition routines capture and record data at user prescribed intervals. The GrowthWorks Software is configurable for running four stimulators while simultaneously monitoring up to 16 transducers, allowing the researcher maximum flexibility.

The GrowthWorks Control Hardware features an embedded In CPU, integrated data acquisition and integrated motor drives. Communications between the laptop and control hardware is transmitted over a standard network cable. Primary advantages of the TGT Laptop/Controller approach are reduced physical size and easy upgradability. As future Windows versions or higher performance Laptops become available, either can be replaced without any software or controller changes. This ensures the GrowthWorks control system will be an integral part of your research for many years to come.

GrowthApps Stiffness Measurement & Adaptive Control:
The GrowthApps modules provided added capability to GrowthWorks. The addition of the Stiffness Measurement & Adaptive Control Application enables the system to make real-time measurements of the sample stiffness during culture. Described in U.S. Patent 7,410,792 B2 Instrumented bioreactor with material property measurement capability and process-based adjustment for conditioning tissue engineered medical products., the Application measures the mechanical response of the tissue construct as it is being stimulated and conditioned. The stiffness measurements can be used as guidelines for bench marking progress during culturing or for modifying the stimulation profile. Based upon the measured response, the bioreactor system is then capable of altering the stimulation profile. This technology enables researchers to automatically culture the tendon or ligament from initial seeding to an implantation-ready state with minimal operator intervention.

LumeGen Bioreactor Configurations
LumeGen bioreactorc configurations range from a single chamber to an integrated six chamber system. The 1V system is a single chamber design that is able to support basic pulsatile pressure and shear stress experiments. Optional features can be added including laser micrometers, pressure sensors and other transducers. The LumeGen 6Vc system integrates up to six chambers,individual reserviors, pumps and stimulator onto a frame that can be easily placed into an incubator. Individual flow loops are provided for each chamber. The table below describes the features of the 1V and 6Vc systems.

Click here to download a LumeGen Brochure （Adobe PDF 144kb）

• 一、美國tissuegrowth公司臨床相關的力學刺激組織工程生物反應器專家，世聯博研北京科技公司作為美國tissuegrowth在中國大陸**代理商，簽約代理以下產品:
• 1、三維力學加載細胞組織工程培養系統
•  
• 2、三維軟骨力學刺激組織工程生物反應器
•  
• 3、3D血管組織工程搏動培養系統
•  
• 4、3D肌腱韌帶組織工程力學培養生物反應器
•  
• 5、3D心臟瓣膜組織工程培養系統
•  
• 6、三維骨應力刺激組織工程生物反應器
•  
• 7、三維皮膚力學機械刺激組織工程生物反應器
•  
• 8、3D灌注培養組織工程生物反應器

• 9、3d支架培養組織工程系統
•  
• 10、多樣品三維力學加載細胞組織組織工程培養系統

• 二、世聯博研北京科技公司還簽約代理美國Flexcell公司細胞拉力、壓力及剪力仿真培養系統
• 是美國Flexcell在中國大陸**代理商，簽約代理以下產品:
• 儀器部分:
• 1、FX-5000C細胞壓力加載系統（flexcell FX5000 Compression system）
• 2、FX-5000T細胞應力加載系統（Flexcell FX5000 Tension system）
• 3、Flex Jr. Tension顯微應力加載系統（Flex Jr. Tension）
• 4、TissueTrain三維細胞組織培養系統（Flexcell Tissue® Train System）
• 5、STR-4000細胞流體切應力系統（Flexcell Fluid Shear Stress Device）
• 6、應力/壓力傳導儀（FlexLink® FX-5000TFL/FX-5000CFL Tension or Compression）
• 7、flexcell自動掃描設備和XyFlEX計算軟件（ScanFlex with XyFlex）
• 8、HiQFlowmate液體控系統（HIQ-4000-A/B/C HiQ Flowmate-Fluid Control System）
• 9、OsciFlow_切應力模式控制器
• 10、SFlex_SFlexJr_顯微應力加載系一號（StageFlexer StageFlexer Jr. Microscopy Tension Device）
• 11、FlexFlow顯微切應力加載設備二號（StageFlexer StageFlexer Microscopy Tension Device）
• 12、STR-4000C 流體剪切系統W/O型電腦（Streamer Fluid Shear System w/o Computer）
• 13、STR-4000D Streamer Fluid Shear Body Only
• 14、Flexcell 儲壓泵（Pressure Reservoir）
• 15、FlexStop真空隔離閥
• flexcell培養耗材部分:
• 1、TTPlate三維細胞培養板
• 2、BioFlex（flexcell BioFlex培養板）
• 5、UniFlex（flexcell雙向應力培養板）
• 6、BioPress壓力加載系統配套使用的BioPress培養板
• 7、細胞培養載片
• 8、圓形TTCircular三維細胞培養板
• 9、HI bioFlex培養板
• 10、SFMembrane_硅膠膜
• Flexcell儀器、配件銷售與租賃

三、世聯博研北京科技公司還簽約美國ebersmedical 一個簡單的機械性刺激細胞培養系統，簽約代理以下產品:

• 1、TC-3 Bioreactor（簡單的機械性刺激細胞培養系統）
• A simple mechanical stimulation cell culture system at last!
• The TC-3 Bioreactor has been designed as a simple easy-to-use system suitable for cell culture
• under mechanical loading profiles defined by the user.
• It has been designed and manufactured thinking of the needs of
• researcher working on cell culture under mechanical stimuli.
• The TC-3 combines the features of a traditional testing machine with the particular demands of cell culture,
• with special emphasis on the parts sterilization, easy assembling,
• sample inspection by microscopy techniques and simple manipulation. Also scalability and
• versatility are key features of the TC-3, in which three grips models can be interchanged
• in order to adapt to the features of the testing substrate or scaffold.
• The system is controlled by a simple computer interface which allows defining the most
• common loading profiles which can be applied on the culture substrate.
• 2、TEB1000 flow bioreactor（TEB1000流體生物反應器）
• 3、TC-3 mechanical stimulation bioreactor（TC-3機械性刺激生物反應器）
• 4、P3D perfusion chambers（灌注細胞培養）
• 5、Vascular Chamber（血博灌注細胞培養）

四、簽約代理美國B-Bridge細胞牽拉儀（STREX）

美國B-Bridge細胞牽拉儀（STREX）是一種模擬細胞生長過程中的機械應力裝置。通過施加細胞應力，張力腔室中的種子細胞被拉伸或壓縮。施加在細胞上的機械應力更好的模擬了自然動態生理環境。STREX可控制施加在細胞上的應力級別，部分型號可與顯微鏡聯用。
ST-140-04
ST-140-10