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EpiQuik總組蛋白抽提試劑盒

,EpiQuik Total Histone Extraction Kit 

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Product Overview

The EpiQuik™ Total Histone Extraction Kit provides a simple and selective method for extracting histone proteins used for a variety of applications, which include histone modification such as acetylation, methylation, and sumoylation. The kit can be used to extract histones from mammalian cells and tissues with the fastest procedure available on the current market, which can be finished within 60 minutes. The EpiQuik™ Total Histone Extraction Kits are also specifically designed to meet the requirements of histone extracts used in EpiQuik™ assays. Yield of the total histone proteins is approximay 0.4 mg per 10⁷ cells or 100 mg of tissue. The yield may vary depending on cell or tissue type.

The EpiQuik™ Total Histone Extraction Kit simply applies our proprietary histone isolation buffers to cells/tissues. After treatment with pre-lysis, lysis, and balance buffers, the total histones are easily extracted for immediate use or storage at proper conditions.

SCHEMATIC PROCEDURE:
	Histone extracts were prepared from MCF-7 cells using the EpiQuik Total Histone Extraction Kit and acetyl histone H3-K9 was quantified using the EpiQuik Global Acetylated Histone H3-K9 Quantification Kit （Fluorometric） （Cat. No. P-4011）.

10X Pre-Lysis Buffer
Lysis Buffer
Balance Buffer
DTT Solution
User Guide

1. Will the buffer affect the histone modification status such as acetylation or methylation?
No, it will not.

2. What is the minimal amount of tissue that can be used for extraction?
1 mg, but for the best results, we recommend more than 10 mg.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by ing info@epigentek.com along with your contact information and institution name.

Yamamura, Y. et. al. （October 2011）. TNF-a inhibits aquaporin 5 expression in human salivary gland acinar cells via suppression of histone H4 acetylation. J Cell Mol Med. 141（8）:1464-8. PubMed Abstract

Jing, L. et. al. （April 2011）. Effect of the histone deacetylase inhibitors on behavioural sensitization to a single morphine exposure in mice. Neurosci Lett. 494（2）: 169-73. PubMed Abstract

Kawakami, K. et. al. （January 2011）. Long interspersed nuclear element-1 hypomethylation is a potential biomarker for the prediction of response to oral fluoropyrimidines in microsalite stable and CpG island methylator phenotype-negative colorectal cancer.Cancer Sci. 102（1）: 166-74. PubMed Abstract

Hansen, A. et. al. （July 2010）. Development of a drug screening platform based on engineered heart tissue. Circulation Research.107（1）: 35-44. PubMed Abstract

Diehl, F. et. al. （March 2010）. Cardiac deletion of smyd2 is dispensable for mouse heart development. PLoS One. 5（3）: e9748.PubMed Abstract