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Cellix公司推出的整套VenaFluxTM 分析平臺是該公司目前Z受客戶歡迎的一套系統，提供在活細胞水平上微流體活性篩選的完整解決方案。操作簡單，分析效率高，可大大提高藥品的開發和篩選效率。
VenaFlux 系統是*個能進行連續流體細胞分析的半自動化微流體分析系統。配合使用Cellix生物芯片和細胞分析軟件，VenaFlux 系統可檢測粘附到微毛細管上的細胞，微毛細管中覆蓋抗體或者

Cellix公司推出的整套VenaFluxTM 分析平臺是該公司目前zui受客戶歡迎的一套系統，提供在活細胞水平上微流體活性篩選的完整解決方案。操作簡單，分析效率高，可大大提高藥品的開發和篩選效率。
    VenaFlux 系統是*個能進行連續流體細胞分析的半自動化微流體分析系統。配合使用Cellix生物芯片和細胞分析軟件，VenaFlux 系統可檢測粘附到微毛細管上的細胞，微毛細管中覆蓋抗體或者培養有內皮細胞，模擬生理流動產生一定的剪切力。VenaFlux 系統操作簡單，可減少耗時耗錢的動物模型實驗，獲得特異性強，準確性高，重復性好的結果。
 

產品特點

• 廣泛使用于各種細胞懸浮液。
• 生物芯片可選擇用光學顯微鏡做細節研究。
• 微流體泵可方便的控制流體剪切力在0.05到20dyne/cm2范圍內。
• 在分析中可調整剪切力。
• 流體速度可控制在pL/min到mL/min。
• 使用DucoCell 分析軟件（DucoCell Analysis Software），可簡單快速的調節好顯微鏡的各項參數。
• 統計分析生成的圖表（如：細胞粘附性VS.剪切力）可方便的導入Excel文檔。
• 電子機械臂移動生物芯片，清洗細微組件和稀釋細胞樣品。
• VenaFluxTM 系統的軟件同步調節泵，翻譯階段篩選，圖像采集，樣品移動和流體控制，
• 各項操作界面均是用戶友好型，系統又易于操作。

詳細資料，請

 

 

 

he VenaFluxTM Platform is the first semi-automated microfluidic platform capable of executing cell rolling, binding/adhesion and migration studies under shear flow mimicking in vivo flow rates.  

Features:

• Robust and easy to use
• Ideal for use with whole blood for plaet adhesion, aggregation and thrombi formation (thrombosis); biofilm culture ove extended periods of time.
• Easy to study cell adhesion, migration and transmigration - more physiologically relevant than static assays.
• Excellent platform for in vitro studies and running experiments in parallel with animal models.

 

Applications:  

• Functional cell-ligand and cell-cell interaction studies.
• Morphology analysis of cell adhesion and migration.
• Tracking of flowing, rolling and migrating cells.
• IC50 curves easily produced.
• Preclinical drug development and lead optimization.

Specifications

The following specifications relate to the key components of the VenaFlux Platform:  Mirus Evo Nanopump, VenaFluxAssay Softwarea and Kima pump

Specifications relating to Microscopy Workstation, Temperature Control, Cell Analysis Software and PC Control vary depending on user application.  Contact Cellix for further information.

Mirus Evo Nanopump
MutliFlow8	Capable of executing up to 8 assays in parallel in Vena8 biochips resulting in an 8-channel syringe pump
Shear Stress Range for cell suspension	0.05 - 10 dyne/cm2; steps of 0.05 dyne/cm2 (100 mL syringe)
Shear Stress Range for whole blood*	2.25 - 450 dyne/cm2 (1 mL syringe)
Volumetric Flow Rates	100 nL/minute - 20 μL/minute (100 mL syringe)
Dead Volume	600 μL
Sample Volume Increments	Freely adjustable Pulsatile flow option available
Valve Switching Time	30 ms max (at 20oC, 2hz, with air under 10psi pressure)
Working Pressure	30 psi - 2 bars maximum
Linear Velocity Range**	10 μm/s to 10 cm/s
Flow Direction	Reversible
Sample Volume Aspiration Accuracy	±1%
Shear Stress Accuracy	±0.5%
Sample Volume Aspiration Precision	<1% CV
Shear Stress Precision	0.5% CV
External Trigger	2 inputs and 2 outputs externall trigger for better operation with external units and softwares
Software Control	Integrated VenaFlux Assay software
Dimensions	Pump:  84mm (W) x 180mm (D) x 192.5mm (H) MultiFlow8:  140mm (W) x 35mm (D) x 140mm (H)
Weight	Pump:  ~2kg MultiFlow8:  <0.5kg

*Considering human whole blood with a viscosity of 4.5 cP

**Given for the flow of distilled water in a microcapillary with dimensions:  400 mm (W) x 100 mm (D) x 20 mm (L)

 

VenaFluxAssay Software

Execution of preset protocols, simultaneous control of pumping, scanning and image acquisitions, sample transfer and protocol flow.  Intuitive stepwise protocol interface with steps hierarchy and status control. Office2007 GUI interface, preset and user-designed protocols.

 

Kima pump
Volumetric Flow Rates	Perfusion mode:  15 - 35 mL/hr (*) +/- 4%
Shear Stress Range	Perfusion mode:  ~2.5 to ~6.5 dyne/cm2(**)
Dead Volume	< 300 µL
Working Pressure	30 psi - 2 bars maximum
Flow Direction	Dispense only. Two modes:  wash and perfusion (controlled only by Kima controller via iPod Touch / iPhone)
Additional features	Plug and play with Kima controller and iKima Application (available for iPod Touch / iPhone) Featured with standard 100mL bottle holder
Dimensions	190mm x 85mm x 90mm (L x W x H)
Weight	< 800g (bottle and tubing not included)
Conditions of use	Temperature: 10?C to 60?C  Humidity: < 98% RH  Altitude: < 2000m

(*): calibrated test made with a&