技術文章

基底硬度細胞牽張拉力影響，基底剛度細胞牽張拉伸刺激

閱讀：1138 發布時間：2019-12-26

基底硬度對細胞牽張拉力影響

CellSoft™ Soft Substrate References

Key References:

Buxboim et al., How deeply do cells feel:methods for thin gels. J Phys Condens matter. 22: 1-19, 2010.‍

Chodhury et al., Soft substrates promote homogeneous self-renewal of embryonic stem cells via down regulating cell-matrix tractions. PLOS 1 5: 1-10, 2010.‍

Engle et al., Matrix elasticity directs stem cell lineage specification. Cell 126: 677-689, 2006.

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Hirata and Yamaoka. Effect of stem cell niche elasticity/ECM protein on the self-beating cardiomyocyte differentiation of iPS cells at different stages. Acta Biomat. 65: 44-52, 2018

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Megone et al., Impact of surface adhesion and sample heterogeneity on the multiscale mechanical characterization of soft biomaterials. Sci Rep 8: 6780, 2018.

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Ou et al., Visualizing mechanical modulation of nanoscale organization of cell-matrix adhesions. Integr. Biol. 8: 795-804,2016.

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Palchesko et al., Development of polydimethylsiloxane substrates with tunable elastic modulus to study cell mechanobiology in muscle and nerve. PLOS 1 7: 1-13, 2012

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Smith et al., Mechanosensing of matrix by stem cells: from matrix heterogeneity, contractility and the nucleus in pore-migration to cardiogenesis and muscle stem cells in vivo. Sem Cell Devtbio. 71: 84-98, 2017

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Solon et al., Fibroblast adaptation andstiffness matching to soft elastic substrates. Biophys J. 93: 4453-4461, 2007.

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Thomas et al., Measuring the mechanical properties of living cells using atomic force microscopy. J Visual Exp. 76:1-8, 2013.

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Vertelov et al., Rigidity of silicone substrates controls cell spreading and stem cell differentiation. Sci Rep. 6:1-10, 2016.

flexcell FX-6000T基底硬度細胞應力刺激加載系統

2D/3D細胞或組織靜態或動態牽張等邊軸或單軸向牽張應變加載和實時觀察
新增不用基底硬度牽張
FX-5000 Tension 系統組成部分:
• 一臺預裝FlexSoft®FX-5000細胞力學加載分析軟件的計算機
• 一臺FX5K™牽張拉伸應力加載控制傳導儀
• 一個BioFlex真空腔應力加載基板，四個密封墊片
• 一套BioFlex直徑為25mm的BioFlex應力加載平臺
• 四塊BioFlex 膠原蛋白1型包被細胞培養板
• 一個干燥過濾器
• 一對濾水器
• 一卷25英尺藍色Flex In鏈接管(6.4毫米外徑)
• 一卷25英尺無色Flex Out鏈接管（9.5毫米外徑）
• 一卷25英尺牽張拉伸泵鏈接藍管(9.5毫米外徑）
• 一瓶硅膠潤滑油
• 一臺儲壓穩壓儀
• 一臺真空負壓牽張拉伸泵
牽張顯微附屬設備
倒置、正置即可
適用樣品：細胞或者組織（2D、3D）
軸向：二維等邊軸、二維單軸向、三維等邊軸、三維單軸向、三維梯形牽張
牽張模式：真空動力抻拉培養板彈性柔性基底膜或3D水凝膠包埋的細胞
實時觀察：StageFlexer顯微附屬設備可在顯微鏡下觀察牽張作用下反應
方便對照：可控制同一塊培養板部分孔細胞受力與否
多組牽張條件作用對照：可同時運行多個不同壓力大小、不同頻率
不同加載周期程序，方便多組牽張條件對比；
軟件精準調控：對牽張加載周期、張應變大小、頻率、波形智能調控
牽張范圍：0 - 33%
牽張頻率：0.01- 5 Hz
細胞量大，便于后期分析：系統牽張傳導儀支持4塊6孔
（每孔1.2* 105個細胞）或24孔牽張板，可同時兼容4個獨立
的FlexLink牽張加載傳導儀，獨立操作四個不同的實驗程序
支持任何波形種類：如更好地控制在超低或超高應力下形：