化工儀器網
資料下載
恒遠產品文獻:雞GP,GS,GK等指標ELISA試劑盒引用文獻
閱讀：999 發布時間：2023-8-21| 提 供 商 | 上海恒遠生物科技有限公司 | 資料大小 | 1.7MB |
|---|---|---|---|
| 資料圖片 | 下載次數 | 7次 | |
| 資料類型 | PDF 文件 | 瀏覽次數 | 999次 |
| 免費下載 | 點擊下載 |
【文獻標題】Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply and Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes
【作者】Longlong Li ,Mengling Peng ,Chongyang Ge, et.al
【作者單位】南京農業大學（Nanjing Agricultural University）
【文獻中引用產品】
雞糖原磷酸化酶(GP)ELISA試劑盒
雞糖原合成酶(GS)ELISA試劑盒
雞葡萄糖激酶(GK)ELISA試劑盒
雞磷酸果糖激酶-1（PFK-1）ELISA試劑盒
雞丙酮酸激酶（PK）ELISA試劑盒
雞丙酮酸脫氫酶E1(PDH E1)ELISA試劑盒
雞檸檬酸合成酶(CS)ELISA試劑盒
雞烏頭酸酶(ACO)ELISA試劑盒
雞磷酸烯醇式丙酮酸羧激酶（PEPCK）ELISA試劑盒
雞ATP-檸檬酸裂解酶(ACLY)ELISA試劑盒
雞NADH脫氫酶ELISA試劑盒
雞ATP合酶（ATP）ELISA試劑盒
【關鍵詞】Hydroxycitric acid Acetyl-CoA ATP-citrate ? Energy metabolism Primary chicken hepatocytes
【DOI】 10.1159/000481564
【影響因子(IF)】11.7
【出版期刊】《CELLULAR PHYSIOLOGY AND BIOCHEMISTRY》
【產品原文引用】
Measurement of the key glucose metabolic enzyme protein content
Cells were cultured in 6-well plates (2×106 cells per well) and treated with 0, 1, 10 or 50 μM (-)-HCA for 24 h. The cells were harvested and disrupted ultrasonically in ice and then centrifuged at 3000 rpm for 20 min at 4 °C. The supernatants were collected, the protein concentrations of glycogen phosphorylase (GP) (catalog #: E-75175), glycogen synthase (GS) (catalog #: E-75174), glucokinase (GK) (catalog #: E-76111), phosphofructokinase-1 (PFK-1) (catalog #: E-76131), pyruvate kinase (PK) (catalog #: E-76565), pyruvate dehydrogenase (PDH, E1) (catalog #: E-76118), citrate synthase (CS) (catalog #: E-75165), aconitase (ACO) (catalog #: E-75144) and phosphoenolpyruvate carboxykinase (PEPCK) (catalog #: E-75117) were measured using ELISA kits according to the manufactures’ protocols (Shanghai Hengyuan Biological Technology Co., China). The enzyme protein content was normalized to total protein concentration in the sample.
完整版PDF文獻請咨詢在線客服或者聯系我司業務員！
更多公司福利請關注“恒遠生物"微信公眾號！