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人SCD40L試劑盒原理
閱讀：1303 發(fā)布時(shí)間：2012-4-25
Description: The human sCD40L ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD40L. The human sCD40L ELISA is for research use only. Not for diagnostic or therapeutic procedures.
CD40 belongs to the TNF receptor superfamily and is a biochemical marker for the identification of activated plaets of patients with cardiovascular diseases （angina pectoris, infarction, stroke etc.）
A key role of CD40/CD40ligand interactions in immune activation, particularly in T-cell dependent B cell responses is anticipated. CD40 Ligand is expressed on the surface of activated CD4+ T cells, basophils, and mast cells. Binding of CD40L to its receptor, CD40, on the surface of B cells stimulates B-cell proliferation, adhesion and differentiation. A soluble isoform of CD40L has been shown to exist in the circulation. This soluble molecule is a homotrimer of a 18kDa protein exhibiting full activity in B cell proliferation and differentiation assays, is able to rescue B cells from apoptosis and binds soluble CD40.
CD40L is discussed in relation to a potential role in supporting B cell tumors and it has been discovered that the molecular defect in the X-linked Hyper-IgM-Syndrome is targeted to the CD40 L gene, it is functional involved in B cell hybridomas and chronic lymphocytic leukemia as well as several autoimmune diseases.
| Reactivity | Human |
|---|---|
| Sample Volume | 20 uL |
| Suitable Sample Types | cell culture supernatant, serum, plasma （EDTA, citrate, heparin） |
| Sensitivity | 0.06 ng/mL |
| Standard Curve Range | 0.16 - 10.0 ng/mL |
| Components | Aluminium pouch（es） with a Microwell Plate coated with monoclonal antibody to human sCD40L HRP-Conjugate anti-human sCD40L monoclonal antibody Human sCD40L Standard lyophilized, 20 ng/ml upon reconstitution Control high, lyophilized Control low, lyophilized Sample Diluent Assay Buffer Concentrate 20x （PBS with 1% Tween 20 and 10% BSA） Wash Buffer Concentrate 20x （PBS with 1% Tween 20） Substrate Solution （tetramethyl-benzidine） Stop Solution （1M Phosphoric acid） Blue-Dye Green-Dye Adhesive Films Dilution Plates |
| Reported Applications | ELISA |
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保存條件及有效期
1．試劑盒保存：2-8℃。
2．有效期：6個(gè)月
運(yùn)輸條件
低溫運(yùn)輸，用干冰或者冰袋低溫運(yùn)輸。