成人做爰免费视频免费看_成人a级高清视频在线观看,成人a大片在线观看,成人a大片高清在线观看,成人av在线播放,一a一级片,一级黄 中国色 片,一级黄 色蝶 片,一级黄色 片生活片

上海復祥生物科技有限公司

當前位置:上海復祥生物科技有限公司>>細胞庫>>人正常細胞>>HUVECHUV-EC 人臍靜脈血管內皮細胞 CRL-1730

HUV-EC 人臍靜脈血管內皮細胞 CRL-1730

參  考  價:¥1300 - ¥3000 / 件
具體成交價以合同協議為準
  • HUVEC

    型號
  • 其他品牌

    品牌
  • 生產商

    廠商性質
  • 上海市

    所在地

更新時間:2025-07-21 17:55:33瀏覽次數:2428

聯系我時,請告知來自 化工儀器網
供貨周期 一周 規格 T25
貨號 HUV-EC-C 應用領域 醫療衛生,食品/農產品,化工,生物產業,制藥/生物制藥
主要用途 科學研究
HUV-EC-C細胞, 人臍靜脈內皮細胞
ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*培養條件

HUV-EC-C細胞, 人臍靜脈內皮細胞

ATCC® Number:CRL-1730™    Price:$280.00
DeBiosSesignations:HUV-EC-C

rum:afety Level:1

Shipped:frozen

Medium &See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:endothelial

Source:Organ: umbilical vein
Tissue: vascular endothelium
Disease: normal
Cell Type: endothelial


Cellular Products:factor VIII [23284]

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
HUV-EC-C細胞, 人臍靜脈內皮細胞

Applications:transfection host (technology from amaxa)

Tumorigenic:No

DNA Profile (STR):Amelogenin: X

CSF1PO: 11,12

D13S317: 9,11

D16S539: 11,12

D5S818: 11,12

D7S820: 8,12

THO1: 6,9.3

TPOX: 8,11

vWA: 16



Cytogenetic Analysis:This is a hypodiploid human cell line. The modal chromosome number was 45 occurring in 72% of cells counted. The rate of polyploid cells was 15.8%. All cells had monosomic N13 and the subclone with additional monosomic N15 predominates. Other coexisting subclones include those with 46,XX,-11,-13,i(11p),i(11q) and 46,XX,+11,-13 karyotypes. Both X chromosomes appear normal.

Comments:Endothelial Cell Growth Supplement (ECGS) and unidentified factors from bovine pituitary, hypothalamus or whole brain extracts are mitogenic for this line. [23284]

The cells have a life expectancy of 50 to 60 population doublings.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated of F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.1 mg/ml heparin; 0.03-0.05 mg/ml endothelial cell growth supplement (ECGS); adjust to a final concentration of 10% fetal bovine serum.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: Two to three times per week


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020



References:23284: Hoshi H, McKeehan WL. Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture. Proc. Natl. Acad. Sci. USA 81: 6413-6417, 1984. PubMed: 6333682
29192: Zahedi K. Characterization of the binding of serum amyloid P to laminin. J. Biol. Chem. 272: 2143-2148, 1997. PubMed: 8999915
33021: Soker S, et al. Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-endoded domain. J. Biol. Chem. 271: 5761-5767, 1996. PubMed:8621443
























HK-2 人腎近曲小管上皮細胞

CRL-2190 HK-2 人腎近曲小管上皮細胞,

人肺成纖維細胞

CCL-153 HFL1 人肺成纖維細胞,原代細胞

人肝星形細胞

LX-2 人肝星形細胞,$r$nATCC 細胞|細

會員登錄

X

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

X
該信息已收藏!
標簽:
保存成功

(空格分隔,最多3個,單個標簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復您~

以上信息由企業自行提供,信息內容的真實性、準確性和合法性由相關企業負責,化工儀器網對此不承擔任何保證責任。

溫馨提示:為規避購買風險,建議您在購買產品前務必確認供應商資質及產品質量。

撥打電話
在線留言
主站蜘蛛池模板: 午夜性色一区二区三区不卡视频| 欧美日韩国产码高清综合人成| 水蜜桃亚洲一二三四在线| 脔到她哭H粗话H上司1V1| 理论片第1页在线看| 激情综合成人五月天| 亚洲天堂一区二区三区| 久久婷婷的综合色丁香五月| 少妇饥渴无码高潮A片爽爽小说| 欧美久久久久久久久中文字幕| 国产亚洲精品品视频在线| 午夜国产精品视频在线| 欧美日韩av网站| 国产亚洲精品久久久久苍井松| 年轻的妈妈4韩国电影| 免费无码又色又爽的视频软件| 日韩精品人妻中文字幕在线| 校园又色又夹爽又黄的小说| 久久日本片精品AAAAA国产| 红杏亚洲影院一区二区三区| 亚州笫一色惰网站| 日韩理论电影在线看| 和邻居交换做爰伦理| 国产欧美久久一区二区三区| 韩国理论电影在线看| 日本强伦姧人妻无码视频| 女神学生亚洲精品av| 男男互C开荤粗肉尿在里VOX| 国产精品AV色欲蜜臀在线| 欧美日日射| 亚洲欧美日韩中文字幕一区| 久草在线草a免费线看| 91麻豆精品国产综合久久久| 国产欧美在线观看精品一区污| 久久精品美女| 看真人毛片A片水真多| 久草在线精品ac| 在线观看亚洲一区二区| 国产97精品久久久天天A片| 99久久综合精品国产| 韩日三级黄色片|