成人做爰免费视频免费看_成人a级高清视频在线观看,成人a大片在线观看,成人a大片高清在线观看,成人av在线播放,一a一级片,一级黄 中国色 片,一级黄 色蝶 片,一级黄色 片生活片

【文獻標題】Chronic exposure to low environmental concentrations and legal aquaculture doses of antibiotics cause systemic adverse effects in Nile tilapia and provoke differential human health risk

【作者】Samwel M. Limbu，Li Zhou，Sheng-Xiang Sun，et.al

【作者單位】華東師范大學（East China Normal University）

【文獻中引用產品】

魚丙二醛（MDA）ELISA試劑盒

【關鍵詞】Low environmental antibiotics，Legal aquaculture antibiotics，Toxicological effects，Human health risk

【DOI】doi.org/10.1016/j.envint.2018.03.034

【影響因子(IF)】8.58

【出版期刊】《Environment International》

【產品原文引用】

After weight measurements and counting, eight fish from each tank were sampled, individually anesthetized with MS-222 (20 mg/L) (tricaine methanesulfonate, Western Chemicals, Inc., Ferndale,Washington) and sacrificed for tissue samples collection for biochemical assays, nutrients body composition and digestibility measurements.Intestines, livers and skins from O. niloticus were excised and subsequently homogenized in a glass beds with 9 volumes (v/w) of 0.8% physiological saline. The homogenate was centrifuged (2500 rpm, at 4 °C for 10 min), the resulting supernatant was collected and subsequently frozen at −80 °C for biochemical assays according to manufacturers' instructions. One whole intestine, liver and a piece of skin from each fish were used in all analyses. On the days of analyses,malondialdehyde (MDA) concentration was determined by using enzyme-linked immune sorbent assay (ELISA) kit (Hengyuan, Shanghai).Protease, lipase, amylase, lactate dehydrogenase (LDH), glutathione Stransferase (GST), superoxide dismutase (SOD), total antioxidant capacity (TAC), acid phosphatase (ACP), and alkaline phosphatase (AKP)were measured by using specific commercial assay kits (Nanjing Jiancheng Institute, China), with their results recorded on a microplate reader (Epoch, BioTek, USA). The remaining fish (with viscera removed) were used for body composition and apparent nutrients digestibility measurements according to standard methods (AOAC, 1995).

完整版PDF文獻請咨詢在線客服或者聯XI我司業務員！

更多公司福利請關注“恒遠生物”VX公眾號！