成人做爰免费视频免费看_成人a级高清视频在线观看,成人a大片在线观看,成人a大片高清在线观看,成人av在线播放,一a一级片,一级黄 中国色 片,一级黄 色蝶 片,一级黄色 片生活片

上海起發實驗試劑有限公司

中級會員·16年

聯系電話

15921799099

您現在的位置: 首頁> 技術文章 > antibodyresearch 113004 說明書
中級會員·16年
人:
楊經理
話:
021-50724187 021-50724961
機:
15921799099
真:
021-50724961
址:
上海浦東川沙鎮川沙路6619號上海起發實驗試劑有限公司
化:
www.qfbio.com
址:
www.qfbio.com

掃一掃訪問手機商鋪

antibodyresearch 113004 說明書

2017-7-27  閱讀(5633)

分享:

HyMAXHybridoma Fusion & Cloning Supplement 20x

貨號: 113004                  

規格:  100mL

 

 

產品描述:

HyMAX™雜交瘤融合與克隆補充劑20x

貨號: 113004
 

替代名稱:雜交瘤補充劑,雜交瘤克隆因子,HCF,雜交瘤克隆補充物,HCS,雜交瘤克隆融合補體,HCFS,雜交瘤生長補充劑,HGS,雜交瘤融合和克隆加強,雜交瘤融合和克隆補充,HFCS,細胞培養雜交瘤媒介補充
說明:HyMax™雜交瘤融合和生長補充劑是含有各種細胞因子,生長因子和激素的條件培養基的專有混合物。在新的雜交瘤發育過程中配制成替代飼養細胞。它也可用于從液氮快速回收冷凍細胞。
融合后細胞電鍍期間常規使用飼養細胞。通常,飼養細胞為新開發的克隆提供許多未知的生長因子。這些包括許多涉及同種型轉換和B細胞IgG分泌維持的細胞因子。從我們多年的雜交瘤發展經驗,我們已經配制了這種有條件的媒體,支持更好的生長和更高頻率的IgG生產克隆開發。測試雜交瘤集落形成效率和IgG分泌的維持。
用法:加入5mL HyMax™20x至95mL您目前用于培養細胞的工作介質,使其占5%。不要使用超過10%的HyMax™。繼續用這種培養基培養細胞,直到細胞生長好幾代。一旦細胞生長良好,通過逐步將百分比逐步降低到1%,0.5%和0%,可以將培養物從HyMax隔離
格式:作為無菌過濾的組織培養物測試,20x濃縮液準備使用溶液。運送到客戶之前切勿凍結。每個100mL瓶子足以制作2L工作介質,足以完成一個完整的雜交瘤開發項目。
儲存:建議在4°C至8°C的冰箱中儲存,直至6個月內使用。它可以制成等分試樣,并在-20°C - 80°C下冷凍保存,長期在12個月內使用。避免反復凍融,這可能會導致活動減少或降水。
穩定性:儲存于冰箱中的4°C至8°C或冷凍儲存12個月時6個月。一旦在30天內解凍使用,或根據需要進行等分和儲存。不要重復凍結和解凍。
運送:準備新鮮(30天內),并以藍色冰袋液體形式運送。

HyMax與其他產品的第三方獨立比較:

 

圖-1用于產生IgG產生B細胞雜交瘤的克隆因子補充物的分析。脾細胞與SP2 / 0細胞融合,培養14天,然后分析來自培養物的上清液。通過抗Fc(γ)ELISA進行分析。IgG陽性克隆被鑒定為產生超過25ng / mL IgG的那些。

 

特點

  • 無血清生長添加劑
  • 低蛋白含量
  • 成分*已知
  • zui高克隆效率
  • 優良的單克隆抗體生產
  • 無需喂養細胞

 

 

品名

貨號

規格

品牌

Hybridoma Fusion and Cloning Supplement; (HFCS)

11363735001

10 ml (50x)

Roche Applied Science

Roche Applied Science品牌的 11363735001 已停產

品名貨號規格品牌
HyMAX™Hybridoma Fusion & Cloning Supplement 20x113004100mLantibodyresearch
HyMAX™Hybridoma Fusion & Cloning Supplement 20x11300425mlantibodyresearch

 

HyMAX™ Hybridoma Fusion and Cloning Supplement, 20X
Catalog No.: 113004
Alternate Names: Hybridoma supplement, Hybridoma cloning factor, HCF, hybridoma cloning supplement, HCS, hybridoma cloning fusion supplement, HCFS, hybridoma growth supplement, HGS, hybridoma fusion and cloning booster, hybridoma fusion and cloning supplement, HFCS, Cell Culture Hybridoma Media Supplements
Description: HyMax™ Hybridoma Fusion and Growth Supplement is a proprietary blend of conditioned media containing various cytokines, growth factors & hormones. It is formulated to replace feeder cells during new hybridoma development. It can also be used for fast recovery of frozen cells from liquid nitrogen.
Feeder cells are routinely used during plating of cells after fusion. Typically feeder cells provide many unknown growth factors to the newly developing clones. These includes many cytokines which are involved in isotype switching and maintenance of IgG secretion by B-cells. From our many years of experience with hybridoma development we have formulated this conditioned media that support better growth & higher frequency of IgG producing clone development. It is tested for hybridoma colony forming efficiency and maintenance of IgG secretion.
Usage: Add 5mL of HyMax™ 20x to 95mL of your working media currently used for culturing cells to make 5%. Do not use HyMax™ at more than 10%. Continue with culturing cells with this media until cells have grown well for few generations. Once cells have grown well, culture may be weaned off of HyMax by gradually reducing the percentage in steps to 1%, 0.5% and 0%.
Format: Provided as a sterile filtered, tissue culture tested, 20x concentrate ready to use solution. Never frozen before shipping to customer. Each 100mL bottle is enough to make 2L of working media, enough for doing a complete hybridoma development project.
Storage: It is recommended to store at 4°C - 8°C in refrigerator until use within 6 months. It can be made into aliquots and frozen stored at -20°C - 80°C for long term to use within a 12 month period. Avoid repeated freezing and thawing, which may cause activity loss and or show precipitation.
Stability: 6 months when stored at 4°C - 8°C in refrigerator or 12 months when stored frozen. Once thawed use within 30 days or aliquot and store as needed. Do not freeze & thaw repeatedly.
Shipping: It is prepared fresh (within 30 days) and shipped in liquid form on blue ice packs.

Third party independent comparisons of HyMax to other products:

 

Figure-1 Analysis of cloning factor supplements for production of IgG-producing B cell hybridomas. Splenocytes were fused with SP2/0 cells, cultured for 14 days before analysis of supernatant from the cultures. Analysis was performed via anti-Fc (gamma) ELISA. IgG positive clones were identified as those producing more than 25ng/mL IgG.

 

我們公司zui大優勢是強大的采購,

1:基本什么都能進口,血清,抗體,耗材,還有部分限制進口的,

2:貨品全,現經營過700多個品牌,基本所有生物試劑耗材都可以進口,特別是冷偏的產品那就更有優勢,

3:提供加急服務,一般1-2周到貨,超過時限加急費全免

4:價格公道,絕大部分價格有優勢,當然不能保證100%產品都是,因為意味著沒有服務.

5:良好的信譽,大部分客戶我們提供貨到付款服務,客戶包括清華,北大 交大 復旦,中山等100多所大學,ROCHE,阿斯利康,國藥 ,fisher等500多家公司

6:我們*代理的品牌有:Antibody Research Corporation,arcticzymes,Biorelevant,AmberGen, Inc. ,clemente-associates,clodronaiposomes,Columbia Biosciences,enzyme research,Gene Bridges GmbH,Genovis,AmberGen, Inc.  Biotechnology GmbH,Haematologic Technologies HTI Haemtech,hookelabs,Immudex,Innovative Research of America,inspiralis ,List Biological Laboratories, Inc.,lumafluor,Microsurfaces,multiplicom,nanotools,Pel-Freez Biologicals,pentapharm,progen,Protein Ark,QA-Bio,Inc,QA-Bio,IncQuickZyme Biosciences,Teknova,TriLink BioTechnologies, Inc.,Zyagen Laboratories 等

7:我們還是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批發,歡迎合作。

會員登錄

×

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

X
該信息已收藏!
標簽:
保存成功

(空格分隔,最多3個,單個標簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復您~
產品對比 二維碼 在線交流

掃一掃訪問手機商鋪

對比框

在線留言
主站蜘蛛池模板: 伦理片福利视频云播放| 久久99精品久久久久久园产越南| 国产国拍亚洲精品av麻豆| 产精品视频在线观看免费| 亚洲国产欧美一区| 国产精一品亚洲二区在线播放| 色人阁久久| 亚洲熟女乱综合一区二区在线| 边吃奶边狠狠躁日韩A片| 国产亚洲欧美日韩精品怀孕| 色综合精品无码一区二区三区| 永久免费毛片| 国产成人亚洲精品无码车A| 成片一卡二卡3卡4卡| 客厅乱H伦亲女小说| 无码又爽又刺激A片涩涩动漫| 国产女人毛片好多水| 亚洲欧美日韩国产另类电影| 性生潮久久久不久久久久| 激情做a全过程片A| 久久性视频| 久久香蕉国产线熟妇人妻| 91久久亚洲综合精品日本| 15部卖最好的AV作品| 一区二区欧美日韩国产| 99精品国产免费久久久久久下载| 亚洲精品中文字幕无码A片老网站| 92午夜福利轻云观看| 羞耻美人妻HD中字电影| 高清国产一区| 国产欧美亚洲精品a国产| 欧美日韩二区高清| 久久久久久久一线毛片| 中文字幕人妻高清| 精品丰满人妻AV久久久| 漂亮人妻被强了中文字幕久久| 中文字幕五月久久婷热| 中文字幕一区二区人妻电影丶| 日本人妻仑乱少妇A级毛片潘金莲| 国产人妻无码精品| 中文字幕有码系列|