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核固紅染色液(0.2%)

Nuclear Fast Red
CAS Number: 6409-77-4
Storage Temperature: Room Temperature
Product Description :
Appearance: Dark Red to Very Dark Brown Powder
Molecular Formula: C14H8NNaO 7S
Molecular Weight: 357.3
Synonyms: Kernechtrot, Calcium Red
This dye has been used as a counterstain in saturated aqueous potassium alum and in a counterstain
solution of 0.1% (w/v) with 5% aluminum sulfate for a Prussian Blue Stain for ferric iron. It has been used
as a reagent for calcium in tissue where it forms a scarlet lake. Lakes are also formed with lead, ferric
ion, copper, tin, and strontium, but not barium. The suitability of nuclear fast red as a nuclear stain in
autoradiographs of knee joints of mice and rats has been reported.
Storage/Stability :
When stored at room temperature, this product is given a shelf-life of four years from the date of
manufacture.
Preparation Instructions:
This dye is reported to be soluble in water (6 mg/ml) and in ethanol (0.4 mg/ml), but it is likely that heat will
be required for the aqueous solution.
Procedure :
Preparation of a nuclear fast red (Kernechrot) solution for use as a counterstain:
1. Dissolve 25 g of aluminum sulfate in 500 ml of distilled water.
2. Add 0.5 g of nuclear fast red. Use heat to dissolve.
3. Cool, filter, and add a few grains of thymol as a pretive.
For use as a counterstain:
1. Rinse sections in distilled water.
2. Counterstain sections in nuclear-fast red solution for 5 minutes.
3. Wash in running tap water for 1 minute or more.
4. If using an aqueous mounting medium, the slides are ready to coverslip. If using a permanent
mounting medium, dehydrate in 2 changes of 95% alcohol, 2 changes of absolute ethanol, and 2 changes
of xylene. Slides can now be mounted using a synthetic, permanent mounting medium.

Note: Inadequate washing of the slides after nuclear fast red staining will result in cloudy slides. If the
slides have not been coverslipped in a permanent mounting medium, return to running water and wash
well. If slides have been coverslipped in a permanent mounting medium, soak in xylene to remove thecoverslip, soak in 3 changes of xylene to remove the mounting