您好, 歡迎來到化工儀器網(wǎng)！ 登錄| 免費注冊| 產(chǎn)品展廳| 收藏商鋪|
您好, 歡迎來到化工儀器網(wǎng)！ 登錄| 免費注冊| 產(chǎn)品展廳| 收藏商鋪|
18906011628
當(dāng)前位置：> 供求商機> VEGF Human, HEK/重組人血管內(nèi)皮生長因子,HEK
| Synonyms | Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609. |
| Introduction | Vascular endothelial growth factor is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types （e.g. stimulation monocyte/ macrophagemigration, neurons, cancer cells, kidney epithelial cells ）.VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesisand cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor. Elevated levels of this protein are linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. |
| Description | Vascular Endothelial Growth Factor Human Recombinant produced in HEK293 cells is a double, glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 40 kDa. The VEGF is purified by proprietary chromatographic techniques. |
| Source | HEK293 （Human Embryonic Kidney cell line）. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | The protein was lyophilized from a concentrated （1mg/ml） solution with no additives. |
| Solubility | It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor-HEK in sterile 18M?-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Vascular Endothelial Growth Factor HEK although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF HEK should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. |
| Amino Acid Sequence | APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Biological Activity | Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells （HUVEC） using a concentration range of 1.0-6.0 ng/ml, corresponding to a specific activity of 1 x 1,000,000 Units/mg to 1.7 x 100,000 Units/mg. |
| Protein content | Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.2875 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a calibrated solution of VEGF as a Reference Standard. |
| Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
請輸入賬號
請輸入密碼
請輸驗證碼
以上信息由企業(yè)自行提供，信息內(nèi)容的真實性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負責(zé)，化工儀器網(wǎng)對此不承擔(dān)任何保證責(zé)任。
溫馨提示：為規(guī)避購買風(fēng)險，建議您在購買產(chǎn)品前務(wù)必確認供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。
