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22322-PIERCE,(SULFO-SMCC) SULFOSUCCINIMIDYL4-(N-MALEIMID
  • 22322-PIERCE,(SULFO-SMCC) SULFOSUCCINIMIDYL4-(N-MALEIMID
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貨物所在地:上海上海市

更新時間:2025-03-12 21:00:31

瀏覽次數:91

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貨號:22322,名稱:(SULFO-SMCC) SULFOSUCCINIMIDYL4-(N-MALEIMIDOMETHYL)CYCLOHEXA ,品牌:PIERCE,規格:50 MG.
.

訂貨:

 

(SULFO-SMCC) SULFOSUCCINIMIDYL4-(N-MALEIMIDOMETHYL)CYCLOHEXA介紹

 

Thermo Scientific BCA蛋白定量產品可以用于研究蛋白:蛋白間相互作用,衡量親和層析后的組分含量,估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

 

(SULFO-SMCC) SULFOSUCCINIMIDYL4-(N-MALEIMIDOMETHYL)CYCLOHEXA現貨供應

 

PIERCE

22322

(SULFO-SMCC) SULFOSUCCINIMIDYL4-(N-MALEIMIDOMETHYL)CYCLOHEXA

50 MG

PIERCE

22324

Sulfo-GMBS, N-gamma-maleimidobutyryloxysulfosuccinimide Este

50 MG

PIERCE

22330

1,6-BISMALEIMIDOHEXANE, BMH

50 MG

 

(SULFO-SMCC) SULFOSUCCINIMIDYL4-(N-MALEIMIDOMETHYL)CYCLOHEXA品牌介紹

 

美國Pierce公司(www.pierce。。com)是蛋白質化學、免疫學和層析等很多方面的和*。http://www.thermo。。com.cn/Category828.html

 

主要產品包括:

1)化學發光體和比色底物

 

2)分析試劑

 

3)熒光標記探針

 

4)酶聯免疫分析試劑盒等

 

(SULFO-SMCC)SULFOSUCCINIMIDYL4-(N-MALEIMIDOMETHYL)CYCLOHEXA僅用于科研

 

  •  

Thermo Scientific Pierce Sulfo-SMCC is a very popular, water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms).

Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC) is a water-soluble, non-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC and SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to ~10 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.

Sulfo-SMCC Highlights

  • Amine reactive Sulfo-NHS ester crosslinks rapidly with primary amine-containing molecule
  • Sulfhydryl-reactive maleimide reacts with cysteine residues to yield specific conjugates
  • Sulfo-SMCC is water soluble, so crosslinking can be done in physiologic solutions
  • High purity, crystalline Sulfo-SMCC can be used to create high-purity maleimide-activated derivatives
  • Cyclohexane bridge confers added stability to the maleimide group making Sulfo-SMCC the ideal crosslinking agent for maleimide activation of proteins. Maleimide groups are stable for 64 hours in 0.1 M sodium phosphate buffer, pH 7 at 4°C.

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