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ODN 2395 VacciGrade™

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  • 公司名稱 深圳欣博盛生物科技有限公司
  • 品牌 Invivogen
  • 型號(hào)
  • 產(chǎn)地 法國(guó)
  • 廠商性質(zhì) 代理商
  • 更新時(shí)間 2022/1/28 17:11:10
  • 訪問(wèn)次數(shù) 1081
產(chǎn)品標(biāo)簽

ODN 2395 VacciGrade™ODN 2395VacciGrade™

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       欣博盛生物科技有限公司是一家致力于生命科學(xué)和生物技術(shù)領(lǐng)域的高科技企業(yè)。公司由在國(guó)內(nèi)科研試劑領(lǐng)域有著十幾年從業(yè)經(jīng)驗(yàn)的專業(yè)技術(shù)團(tuán)隊(duì)和企業(yè)管理團(tuán)隊(duì)組建而成,專門從事以抗體、細(xì)胞因子、免疫檢測(cè)試劑盒等免疫學(xué)產(chǎn)品為主的生物試劑的研發(fā)與銷售。公司代理40余家歐美*生物技術(shù)公司的相關(guān)產(chǎn)品,能為生命科學(xué)多研究領(lǐng)域的廣大科研人員提供數(shù)萬(wàn)種抗體、數(shù)千種蛋白以及相關(guān)檢測(cè)試劑盒等。經(jīng)過(guò)幾年的不懈努力,欣博盛生物科技有限公司已成為免疫學(xué)、細(xì)胞生物學(xué)、分子生物學(xué)等領(lǐng)域科研產(chǎn)品的*供應(yīng)商。同時(shí),公司自主研發(fā)的ELISA試劑盒,品質(zhì)*,行業(yè)*;在國(guó)內(nèi)眾多重點(diǎn)實(shí)驗(yàn)室廣泛使用,深受廣大科研人員的好評(píng)。欣博盛品牌ELISA試劑盒自推出以來(lái)已先后在Nature,PNAS, Cell research等雜志文章中被引用。

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   我們的一切努力和付出,惟愿助您一臂之力!

 

 

 

 

ELISA試劑盒,抗體,蛋白/肽,分子化合物,IVD原料,特色試劑盒,其他試劑,耗材

供貨周期 現(xiàn)貨 規(guī)格 1 mg
貨號(hào) vac-2395-1 應(yīng)用領(lǐng)域 生物產(chǎn)業(yè)
主要用途 科研

CpG ODN, class C (human / mouse) - TLR9-based adjuvant

ODN 2395 is a synthetic oligodeoxynucleotide containing unmethylated CpG motifs (CpG ODNs).CpG ODNs are recognized by TLR9, which is expressed exclusively on human B cells and plasmocytoid dendritic cells (pDCs), thereby inducing Th1-dominated immune responses.

ODN 2395 is a class C CpG ODN specific for mouse TLR9. In vivo studies have demonstrated that C-class ODNs, such ODN 2395, are very potent Th1 adjuvants.

ODN 2395 VacciGrade™ is suitable for preclinical studies. It is prepared under strict aseptic conditions. It is guaranteed sterile and thoroughly tested for the presence of endotoxins.

ODN 2395 VacciGrade™ is for research use only, not for use in humans.


Specifications

Specificity: Th1 response, human / mouse.

Quality: Sterile, Endotoxin level <1 EU/mg.

Working concentration: 20-50 µg/mouse.

ODN 2395 sequence: 5’-tcgtcgttttcggcgc:gcgccg-3’ (22 mer)

Quality control:

  • ODN 2395 VacciGrade™ is a preclinical grade preparation of the CpG ODN 2395.

  • It is prepared under strict aseptic conditions and is tested for the presence of endotoxins.

  • ODN 2395 VacciGrade™ is guaranteed sterile and its endotoxin level is <1 EU/mg.


Contents

  • 1 mg (141.85 nmol) of sterile lyophilized ODN 2395 VacciGrade™

  • 10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

 ODN 2395 VacciGrade™ is shipped at room temperature

 Should be stored at -20°C for up to 1 year.
Upon resuspension, prepare aliquots of ODN 2395  VacciGrade™ and store at -20°C.

 Product is stable 6 months at -20°C when properly stored.

 Avoid repeated freeze-thaw cycles.


Details

Synthetic oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODNs) have been extensively studied as adjuvants [1]. These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA [2].
CpG ODNs are recognized by TLR9, which is expressed exclusively on human B cells and plasmacytoid dendritic cells (pDCs), thereby inducing Th1-dominated immune responses [3].
Pre-clinical studies, conducted in rodents and non-human primates, and human clinical trials have demonstrated that CpG ODNs can significantly improve vaccine-specific antibody responses [1].

Three types of stimulatory CpG ODNs have been identified, types A, B and C, which differ in their immune-stimulatory activities [4-5]. Furthermore, CpG ODNs activate TLR9 in a species-specific manner [6].

ODN 2395 is a type C CpG ODN specific for mouse TLR9 [7]. Type C CpG ODNs contain a full phosphorothioate backbone with one or more CpG dinucleotides. Type C CpG ODNs are potent inducers of IFN-α from pDC and strong B cell activators [8, 9]. In vivo studies have demonstrated that type C ODNs which combine the effects of types A and B ODNs, such ODN 2395, are very potent Th1 adjuvants [10].

 

1. Steinhagen F. et al., 2011. TLR-based immune adjuvants. Vaccine 29(17):3341-55.
2. Hemmi H. et al., 2000. A Toll-like receptor recognizes bacterial DNA. Nature 408: 740-5.
3. Coffman RL. et al., 2010. Vaccine adjuvants: Putting innate immunity to work. Immunity 33(4):492-503.
4. Krug A. et al., 2001. Identification of CpG oligonucleotide sequences with high induction of IFN-alpha/beta in plasmacytoid dendritic cells. Eur J Immunol, 31(7): 2154-63.
5. Marshall JD. et al., 2005. Superior activity of the type C class of ISS in vitro and in vivo across multiple species. DNA Cell Biol. 24(2):63-72.
6. Bauer S. et al., 2001. Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition. PNAS, 98(16):9237-42.
7. Moseman EA. et al., 2004. Human plasmacytoid dendritic cells activated by CpG oligodeoxynucleotides induce the generation of CD4+CD25+ regulatory T cells. J Immunol. 173:4433-4442.
8. Jurk M. et al., 2004. C-Class CpG ODN: sequence requirements and characterization of immunostimulatory activities on mRNA level. Immunobiology. 209(1-2):141-54.
9. Abel K. et al., 2005. Deoxycytidyl-deoxyguanosine oligonucleotide classes A, B, and C induce distinct cytokine gene expression patterns in rhesus monkey peripheral blood mononuclear cells and distinct alpha interferon responses in TLR9-expressing rhesus monkey plasmacytoid dendritic cells. Clin Diagn Lab Immunol. 12(5):606-21.
10. Vollmer J. et al., 2004. Characterization of three CpG oligodeoxynucleotide classes with distinct immunostimulatory activities. Eur J Immunol. 34(1):251-62.


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